| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1271 | Methanosphaera | GGGGAACACCACTCATGCT | TCCCAGTATTTGTGCTGCTTC | 59.62 | 58.56 | 203 | 61 |
100.00%
|
66.67%
|
| Primer 1272 | Methanosphaera | TTGGAACAGCAGCTGCACT | TCTTGATCTGGCCATGTTGCT | 60.15 | 59.99 | 253 | 61 |
100.00%
|
100.00%
|
| Primer 1273 | Methanosphaera | GGAACAGCAGCTGCACTTG | TCTTGATCTGGCCATGTTGCT | 59.71 | 59.99 | 251 | 61 |
100.00%
|
66.67%
|
| Primer 1274 | Methanosphaera | CATGCATGTAACGGCAGGT | TTGTGGTCCAGTTTGTCCCA | 58.52 | 59.37 | 205 | 61 |
100.00%
|
66.67%
|
| Primer 1275 | Methanosphaera | GGAATTCCAAGGGGTGTAGGT | AAACATGCAGTTCCAGGTTGC | 59.36 | 59.93 | 287 | 61 |
100.00%
|
66.67%
|
| Primer 1276 | Methanosphaera | TTGGCTGTGATCCTAAGGCT | ACACCCCTACCAGCACAAC | 58.71 | 59.55 | 187 | 61 |
100.00%
|
66.67%
|
| Primer 1277 | Methanosphaera | GGTGGTGGCGACTTAGCAA | TACTTGCAACCCAACATGCT | 60.30 | 58.01 | 286 | 61 |
100.00%
|
66.67%
|
| Primer 1278 | Methanosphaera | ATGGTGGTGGCGACTTAGC | TTACTTGCAACCCAACATGCT | 60.08 | 58.68 | 289 | 61 |
100.00%
|
66.67%
|
| Primer 1279 | Methanosphaera | GCAGACAAAGCCTCAGAAGAA | ACGTTTTGGTGGATGGGGA | 58.50 | 59.15 | 210 | 61 |
100.00%
|
66.67%
|
| Primer 1280 | Methanosphaera | GGTCCAACACCTCTTGCATC | TGTATTTAGAAGCGCAGGGGT | 58.83 | 59.44 | 177 | 61 |
100.00%
|
66.67%
|