| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1391 | Methanosphaera | TCCATGAACACAAACCCCAAC | TCCACATGGTGCTGGTGTT | 58.96 | 59.46 | 242 | 55 |
100.00%
|
66.67%
|
| Primer 1392 | Methanosphaera | ACGCCTTCTGAAGCAGGA | TCCAGCTACAACTCCCACCT | 58.53 | 60.18 | 297 | 55 |
100.00%
|
66.67%
|
| Primer 1393 | Methanosphaera | ACTTGTAGGCGTTGGTGGAA | TCATGGCAGTTCCTGAAACCT | 59.53 | 59.57 | 272 | 55 |
100.00%
|
66.67%
|
| Primer 1394 | Methanosphaera | GCAACTGCCATAGCCCAAG | CCTGTATGACATGCTCCACCT | 59.19 | 59.51 | 245 | 55 |
100.00%
|
66.67%
|
| Primer 1395 | Methanosphaera | GGGGAACACCACTCATGCTA | TCCCAGTATTTGTGCTGCTTC | 59.38 | 58.56 | 203 | 55 |
100.00%
|
66.67%
|
| Primer 1396 | Methanosphaera | CATGCATGTAACGGCAGGTT | TGTGGTCCAGTTTGTCCCA | 59.19 | 58.69 | 204 | 55 |
100.00%
|
66.67%
|
| Primer 1397 | Methanosphaera | GGCTGTGATCCTAAGGCTGA | ACCCCTACCAGCACAACCT | 59.17 | 60.46 | 183 | 55 |
100.00%
|
66.67%
|
| Primer 1398 | Methanosphaera | TTGGCTGTGATCCTAAGGCTG | ACCCCTACCAGCACAACCT | 60.07 | 60.46 | 185 | 55 |
100.00%
|
66.67%
|
| Primer 1399 | Methanosphaera | GGTGGTGGCGACTTAGCAA | ACTTGCAACCCAACATGCTA | 60.30 | 58.01 | 285 | 55 |
100.00%
|
66.67%
|
| Primer 1400 | Methanosphaera | GTGGTAGTGCATGGTGAAACA | TTGCCACATGCCCATTACCT | 58.77 | 59.96 | 199 | 55 |
100.00%
|
66.67%
|