| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1401 | Methanosphaera | ACCCAAGGGCATCAATTGACA | GCATGTCTGCTTGTCCAGTT | 60.20 | 58.76 | 210 | 55 |
100.00%
|
66.67%
|
| Primer 1402 | Methanosphaera | ACTCTGTTGTCGATGCACAAT | CCCCAGGTGTTGAAACTTCCT | 58.23 | 60.13 | 156 | 55 |
100.00%
|
66.67%
|
| Primer 1403 | Methanosphaera | TGGAACACGCATTGCTGTT | CCAAAACACCATACACGCCA | 58.59 | 59.04 | 212 | 55 |
100.00%
|
66.67%
|
| Primer 1404 | Methanosphaera | TGGAACACGCATTGCTGTT | CCAAAACACCATACACGCCAT | 58.59 | 59.46 | 212 | 55 |
100.00%
|
66.67%
|
| Primer 1405 | Methanosphaera | GCCCTAATTGTAGAAGCCACG | TCACAGTCTGCGATTTGTGC | 59.06 | 59.13 | 181 | 54 |
100.00%
|
33.33%
|
| Primer 1406 | Methanosphaera | TGATGAACCAACAAGCGGACT | TGGAAGACGTAGATTTGCACG | 60.20 | 58.67 | 235 | 54 |
100.00%
|
33.33%
|
| Primer 1407 | Methanosphaera | ACCAACAAGCGGACTAGATCC | TGGAAGACGTAGATTTGCACG | 59.79 | 58.67 | 229 | 54 |
100.00%
|
33.33%
|
| Primer 1408 | Methanosphaera | ACGCCAACAGCAGATATGCA | CTCCACGTGTAGCACCATGA | 60.39 | 59.75 | 280 | 54 |
100.00%
|
33.33%
|
| Primer 1409 | Methanosphaera | ACAGGGACGCGAAATAAAAGT | TCCTCCTCCTACAGCATCATC | 58.23 | 58.40 | 289 | 54 |
100.00%
|
33.33%
|
| Primer 1410 | Methanosphaera | GGTGATGAGGCAAAAGCACG | TGCCCATGCATCACCTTGAT | 60.11 | 60.03 | 171 | 54 |
100.00%
|
33.33%
|