| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1421 | Methanosphaera | AAGGCGAAGTCACTGACGA | TACTCGTGGAGGGTGTGCT | 58.96 | 60.23 | 263 | 53 |
100.00%
|
33.33%
|
| Primer 1422 | Methanosphaera | AAGGCGAAGTCACTGACGA | TCGTGGAGGGTGTGCTTT | 58.96 | 58.76 | 260 | 53 |
100.00%
|
33.33%
|
| Primer 1423 | Methanosphaera | TGCAACCTGTTCAGGAGCA | GGTACAGCGTGTCCGGTTT | 59.47 | 60.30 | 279 | 53 |
100.00%
|
33.33%
|
| Primer 1424 | Methanosphaera | GCAGGAGGGCTCTTTGCAT | AGCTGCATCTGCGAAACGA | 60.38 | 60.37 | 165 | 53 |
100.00%
|
66.67%
|
| Primer 1425 | Methanosphaera | GAGGGCTCTTTGCATCAGGT | GCTGCATCTGCGAAACGA | 60.03 | 58.83 | 160 | 53 |
100.00%
|
66.67%
|
| Primer 1426 | Methanosphaera | AGCACTACTATTTGCAGCCGA | TCTGGTCGTGGTGTATGTTTG | 59.79 | 58.24 | 180 | 53 |
100.00%
|
66.67%
|
| Primer 1427 | Methanosphaera | AGCACTACTATTTGCAGCCGA | TGGTCGTGGTGTATGTTTGTT | 59.79 | 58.08 | 178 | 53 |
100.00%
|
66.67%
|
| Primer 1428 | Methanosphaera | GCACTACTATTTGCAGCCGA | TCTGGTCGTGGTGTATGTTTG | 58.35 | 58.24 | 179 | 53 |
100.00%
|
66.67%
|
| Primer 1429 | Methanosphaera | GCACTACTATTTGCAGCCGA | TGGTCGTGGTGTATGTTTGTT | 58.35 | 58.08 | 177 | 53 |
100.00%
|
66.67%
|
| Primer 1430 | Methanosphaera | CGGGAGCTACTATTGGACGA | CCCTCAGGTGCTGGTTTTGT | 58.68 | 60.47 | 262 | 53 |
100.00%
|
66.67%
|