| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1411 | Methanosphaera | TGGCTCCGGATAACACACG | CAGCATCAAAGGTGGCAGT | 59.78 | 58.35 | 186 | 54 |
100.00%
|
33.33%
|
| Primer 1412 | Methanosphaera | AGGTGTGGCATTGGGTGAAA | CAGCCCATGTTACTGCTTCA | 60.11 | 58.17 | 248 | 54 |
100.00%
|
66.67%
|
| Primer 1413 | Methanosphaera | TCACTGGGCTCTACCTGGA | TATGTCCTCTCGGGTCACG | 59.22 | 58.21 | 152 | 54 |
100.00%
|
66.67%
|
| Primer 1414 | Methanosphaera | ACGGGTGGAGCAGGATTT | TCCTCACCATTCCAACGACC | 58.50 | 59.68 | 218 | 53 |
100.00%
|
33.33%
|
| Primer 1415 | Methanosphaera | TGGAACAGCAGCAGCACT | GGTCTGGCCATGTTGCTTT | 59.49 | 58.65 | 248 | 53 |
100.00%
|
100.00%
|
| Primer 1416 | Methanosphaera | AAAAGTTGCAGCAACCCGA | CAGCTCCAAAGAGTGCTGC | 58.50 | 59.13 | 254 | 53 |
100.00%
|
33.33%
|
| Primer 1417 | Methanosphaera | ATCAGCCAAGCCAACAGC | CTTGTAGCTCTGGTGGCGA | 58.31 | 59.41 | 212 | 53 |
100.00%
|
33.33%
|
| Primer 1418 | Methanosphaera | TTTGGACGTGGAGGCGAA | TGGTGTGTCAGCATCACGA | 59.18 | 59.25 | 291 | 53 |
100.00%
|
33.33%
|
| Primer 1419 | Methanosphaera | AGGCGAAGTCACTGACGA | TACTCGTGGAGGGTGTGCT | 58.24 | 60.23 | 262 | 53 |
100.00%
|
33.33%
|
| Primer 1420 | Methanosphaera | AGGCGAAGTCACTGACGA | TCGTGGAGGGTGTGCTTT | 58.24 | 58.76 | 259 | 53 |
100.00%
|
33.33%
|