| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 181 | Methanosphaera | TGGACATCATCACAAATGGGG | TTATCGCGAGCTTGATCTCCT | 58.26 | 59.04 | 190 | 115 |
100.00%
|
50.00%
|
| Primer 182 | Methanosphaera | CCATGAACACAAACCCCAACA | TCCACATGGTGCTGGTGT | 59.24 | 58.75 | 241 | 115 |
100.00%
|
100.00%
|
| Primer 183 | Methanosphaera | CCATGAACACAAACCCCAACA | TTCCACATGGTGCTGGTGT | 59.24 | 59.46 | 242 | 115 |
100.00%
|
100.00%
|
| Primer 184 | Methanosphaera | AGTAGTAGGTGTGGGCATGT | TTCCTGCCTGTGGACTGT | 58.05 | 58.01 | 284 | 115 |
100.00%
|
100.00%
|
| Primer 185 | Methanosphaera | AGTAGTAGGTGTGGGCATGT | TCCTTTTGTAGGCGTGCTGT | 58.05 | 59.89 | 220 | 115 |
100.00%
|
100.00%
|
| Primer 186 | Methanosphaera | AGTAGTAGGTGTGGGCATGT | TTTCCTGCCTGTGGACTGT | 58.05 | 58.77 | 285 | 115 |
100.00%
|
100.00%
|
| Primer 187 | Methanosphaera | ACGCCTTCTGAAGCAGGAG | CCAGCTACAACTCCCACCTG | 59.71 | 60.04 | 296 | 115 |
100.00%
|
100.00%
|
| Primer 188 | Methanosphaera | GGCTGTAAAAGGTCATGGAGC | CGTATGTTCCATCGGCAACA | 59.25 | 58.64 | 203 | 115 |
100.00%
|
100.00%
|
| Primer 189 | Methanosphaera | AGCAGCACACCCGTATTGT | AGCATCACTCGCTCCAATCTC | 59.63 | 60.20 | 172 | 115 |
100.00%
|
100.00%
|
| Primer 190 | Methanosphaera | AGCAGCACACCCGTATTGT | GCATCACTCGCTCCAATCTC | 59.63 | 58.78 | 171 | 115 |
100.00%
|
100.00%
|