| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 201 | Methanosphaera | GCTGGTGCATATATGGGTGGA | GGCCATATACAGCCTCACCA | 59.93 | 59.24 | 160 | 113 |
100.00%
|
66.67%
|
| Primer 202 | Methanosphaera | GGAGACATGACAGGACTTGCA | ACCCACCCCTGTACAACCA | 60.00 | 60.39 | 225 | 113 |
100.00%
|
66.67%
|
| Primer 203 | Methanosphaera | TGGAGACATGACAGGACTTGC | ACCCACCCCTGTACAACCA | 60.00 | 60.39 | 226 | 113 |
100.00%
|
66.67%
|
| Primer 204 | Methanosphaera | CCATGCATGTAACGGCAGG | TGTGGTCCAGTTTGTCCCA | 59.27 | 58.69 | 205 | 113 |
100.00%
|
66.67%
|
| Primer 205 | Methanosphaera | AGGAATACTCACCTCGGGAGT | AGTGAACCACCATGTGCCA | 59.71 | 59.46 | 201 | 113 |
100.00%
|
66.67%
|
| Primer 206 | Methanosphaera | ACGCGAATTTGGTGCACA | AGCACTTTGAGCTCCACCA | 58.57 | 59.16 | 226 | 113 |
100.00%
|
66.67%
|
| Primer 207 | Methanosphaera | AGGCGACGATGCAGATGT | GGGTTTGTCCATCTGCGATG | 59.10 | 59.27 | 191 | 112 |
100.00%
|
33.33%
|
| Primer 208 | Methanosphaera | TGTAAGACGAGACTGGGCAC | GCTGCTATTACGTGTGGTGC | 59.40 | 59.63 | 219 | 112 |
100.00%
|
33.33%
|
| Primer 209 | Methanosphaera | TTGTAAGACGAGACTGGGCAC | GCTGCTATTACGTGTGGTGC | 60.00 | 59.63 | 220 | 112 |
100.00%
|
33.33%
|
| Primer 210 | Methanosphaera | GGAACATGGCGTGCAATGG | CAGCAAGTGGTGACCATGC | 60.15 | 59.42 | 220 | 112 |
100.00%
|
33.33%
|