| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 191 | Methanosphaera | AGCAGCACACCCGTATTGT | ATCACTCGCTCCAATCTCAGG | 59.63 | 59.59 | 169 | 115 |
100.00%
|
66.67%
|
| Primer 192 | Methanosphaera | TGGGGACAACGTCAATTCAGA | TTTCCTGCACGAATGTTGGT | 59.58 | 58.31 | 161 | 114 |
100.00%
|
33.33%
|
| Primer 193 | Methanosphaera | TCAAAGAAGGGGTATGGCCTT | TGCACCTAAGACAACCTGTGA | 59.00 | 59.23 | 234 | 114 |
100.00%
|
66.67%
|
| Primer 194 | Methanosphaera | TTAACGCACCTGACTGGGC | TCCACGGTCTTTGTTGCCA | 60.30 | 59.78 | 176 | 113 |
100.00%
|
33.33%
|
| Primer 195 | Methanosphaera | AGTAACGGGTGGAGCAGGA | CCTCACCATTCCAACGACCA | 60.23 | 59.96 | 221 | 113 |
100.00%
|
33.33%
|
| Primer 196 | Methanosphaera | GTCCTGCACTTGGAGCTGT | TGCTCCTTCTTGGCGATCTAC | 59.93 | 59.86 | 260 | 113 |
100.00%
|
33.33%
|
| Primer 197 | Methanosphaera | CTGTAAGTGCATGGGCTCCA | TCTCCACGTTCTGGTGTGT | 60.04 | 58.49 | 169 | 113 |
100.00%
|
33.33%
|
| Primer 198 | Methanosphaera | TGTAAGTGCATGGGCTCCA | TCTCCACGTTCTGGTGTGT | 58.93 | 58.49 | 168 | 113 |
100.00%
|
33.33%
|
| Primer 199 | Methanosphaera | AGGACCACATGCATGTACTGG | TGATGCCCTCAGCTTCCAC | 60.07 | 59.70 | 188 | 113 |
100.00%
|
33.33%
|
| Primer 200 | Methanosphaera | CAGTTGGCGATGTTGGTGT | TGAGCATAAAGAGCAGCACCA | 58.67 | 60.00 | 169 | 113 |
100.00%
|
66.67%
|