| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Methanosphaera | ACTCCTGCACCACCAGAGT | GGACGTCTAGCCCAAAGTTGT | 60.46 | 60.27 | 269 | 110 |
100.00%
|
50.00%
|
| Primer 222 | Methanosphaera | TTAACGCACCTGACTGGGC | CACGGTCTTTGTTGCCACC | 60.30 | 59.64 | 174 | 110 |
100.00%
|
50.00%
|
| Primer 223 | Methanosphaera | TTAACGCACCTGACTGGGC | CCACGGTCTTTGTTGCCAC | 60.30 | 59.64 | 175 | 110 |
100.00%
|
50.00%
|
| Primer 224 | Methanosphaera | CGTAACCACCCCAAGCAGT | CCTCCATCATCTGCAAGTGC | 59.93 | 58.98 | 180 | 110 |
100.00%
|
50.00%
|
| Primer 225 | Methanosphaera | ACGTTTTCAGTCATTCTGGGC | CCAAGTGCAAGAAGTGGTGC | 59.12 | 59.97 | 186 | 110 |
100.00%
|
50.00%
|
| Primer 226 | Methanosphaera | ACGTTTTCAGTCATTCTGGGC | ACCAAGTGCAAGAAGTGGTG | 59.12 | 58.61 | 187 | 110 |
100.00%
|
50.00%
|
| Primer 227 | Methanosphaera | AGGTGATGAGGCAAAAGCAC | TGCCCATGCATCACCTTGA | 58.75 | 59.62 | 172 | 110 |
100.00%
|
50.00%
|
| Primer 228 | Methanosphaera | TCACGAGAACACACCCAAGT | TTGGGCAGAGCACTTCTCC | 59.17 | 59.62 | 229 | 110 |
100.00%
|
50.00%
|
| Primer 229 | Methanosphaera | TCACGAGAACACACCCAAGT | TTTGGGCAGAGCACTTCTCC | 59.17 | 60.25 | 230 | 110 |
100.00%
|
50.00%
|
| Primer 230 | Methanosphaera | AGTAGTAGGTGTGGGCATGT | TCCTGCCTGTGGACTGTAGT | 58.05 | 60.18 | 283 | 110 |
100.00%
|
100.00%
|