| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 241 | Methanosphaera | AGGCGACGATGCAGATGT | TGGGTTTGTCCATCTGCGAT | 59.10 | 59.67 | 192 | 108 |
100.00%
|
33.33%
|
| Primer 242 | Methanosphaera | GGCTGTAAAAGGTCATGGAGC | CATCGTATGTTCCATCGGCAA | 59.25 | 58.79 | 206 | 108 |
100.00%
|
66.67%
|
| Primer 243 | Methanosphaera | AGGAATACTCACCTCGGGAGT | ACCACCATGTGCCATGGAT | 59.71 | 59.30 | 196 | 108 |
100.00%
|
66.67%
|
| Primer 244 | Methanosphaera | AGGAATACTCACCTCGGGAGT | AACCACCATGTGCCATGGAT | 59.71 | 59.96 | 197 | 108 |
100.00%
|
66.67%
|
| Primer 245 | Methanosphaera | ACTCCTGCACCACCAGAGT | AGAGGACGTCTAGCCCAAAG | 60.46 | 58.81 | 272 | 107 |
100.00%
|
33.33%
|
| Primer 246 | Methanosphaera | GCTGATGGTGCAACAACAGC | TGCACAGCTCTATCCCCATC | 60.67 | 59.24 | 260 | 107 |
100.00%
|
66.67%
|
| Primer 247 | Methanosphaera | TCGTTGGAACAGGCGAAGT | TGTGTGTTGTTTGTTGCAGCT | 59.56 | 59.73 | 202 | 106 |
100.00%
|
33.33%
|
| Primer 248 | Methanosphaera | GGGGACAACGTCAATTCAGAC | TTTCCTGCACGAATGTTGGT | 59.20 | 58.31 | 160 | 106 |
100.00%
|
33.33%
|
| Primer 249 | Methanosphaera | GCAAGCGAACTTAAATGGGGA | TTTCCTGCACGAATGTTGGT | 59.18 | 58.31 | 176 | 106 |
100.00%
|
33.33%
|
| Primer 250 | Methanosphaera | TGGGGACAACGTCAATTCAG | TTTCCTGCACGAATGTTGGT | 58.11 | 58.31 | 161 | 106 |
100.00%
|
33.33%
|