| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 231 | Methanosphaera | AGGAATACTCACCTCGGGAGT | GAACCACCATGTGCCATGG | 59.71 | 59.11 | 198 | 110 |
100.00%
|
66.67%
|
| Primer 232 | Methanosphaera | AGGTGTGGCATTGGGTGA | CAGCCCATGTTACTGCTTCA | 58.74 | 58.17 | 248 | 110 |
100.00%
|
66.67%
|
| Primer 233 | Methanosphaera | TCACTGGGCTCTACCTGGAG | GTATGTCCTCTCGGGTCACG | 60.32 | 59.62 | 153 | 110 |
100.00%
|
66.67%
|
| Primer 234 | Methanosphaera | ATCACTGGGCTCTACCTGGA | GTATGTCCTCTCGGGTCACG | 59.66 | 59.62 | 154 | 110 |
100.00%
|
66.67%
|
| Primer 235 | Methanosphaera | AGGCGACGATGCAGATGT | TGGGTTTGTCCATCTGCGA | 59.10 | 59.24 | 192 | 109 |
100.00%
|
33.33%
|
| Primer 236 | Methanosphaera | TGATTGCACAAGGAGCACGA | TTGGTGCTGGGCTTGCTT | 60.25 | 60.12 | 174 | 109 |
100.00%
|
33.33%
|
| Primer 237 | Methanosphaera | GATTGCACAAGGAGCACGA | TTGGTGCTGGGCTTGCTT | 58.46 | 60.12 | 173 | 109 |
100.00%
|
33.33%
|
| Primer 238 | Methanosphaera | GCAGTAGCATTCATGGATCCA | GTTAGCCCATGGTCCACGA | 58.15 | 59.40 | 165 | 109 |
100.00%
|
33.33%
|
| Primer 239 | Methanosphaera | CCTTGGAGAAAGAAGTCCACG | GGAAAATACGAGGGTCGCGA | 58.58 | 60.18 | 170 | 109 |
100.00%
|
66.67%
|
| Primer 240 | Methanosphaera | AGAAGGGGTATGGCCTTGTTT | TGCACCTAAGACAACCTGTGA | 59.28 | 59.23 | 230 | 109 |
100.00%
|
66.67%
|