| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Methanosphaera | CACCACCAATGCACAACACA | ACAGCTCCTATTTTCTGGGCT | 59.54 | 59.08 | 208 | 128 |
100.00%
|
50.00%
|
| Primer 52 | Methanosphaera | ACCAATGCACAACACAGCA | ACAGCTCCTATTTTCTGGGCT | 58.50 | 59.08 | 204 | 128 |
100.00%
|
50.00%
|
| Primer 53 | Methanosphaera | CCACCAATGCACAACACAGC | ACAGCTCCTATTTTCTGGGCT | 60.60 | 59.08 | 206 | 128 |
100.00%
|
50.00%
|
| Primer 54 | Methanosphaera | TCACCACCAATGCACAACAC | ACAGCTCCTATTTTCTGGGCT | 59.26 | 59.08 | 209 | 128 |
100.00%
|
50.00%
|
| Primer 55 | Methanosphaera | ACCACCAATGCACAACACAG | ACAGCTCCTATTTTCTGGGCT | 59.25 | 59.08 | 207 | 128 |
100.00%
|
50.00%
|
| Primer 56 | Methanosphaera | CACCAATGCACAACACAGC | ACAGCTCCTATTTTCTGGGCT | 58.11 | 59.08 | 205 | 128 |
100.00%
|
50.00%
|
| Primer 57 | Methanosphaera | ACCACCAATGCACAACACA | ACAGCTCCTATTTTCTGGGCT | 58.11 | 59.08 | 207 | 128 |
100.00%
|
50.00%
|
| Primer 58 | Methanosphaera | CTCACCACCAATGCACAACA | ACAGCTCCTATTTTCTGGGCT | 58.97 | 59.08 | 210 | 128 |
100.00%
|
50.00%
|
| Primer 59 | Methanosphaera | CTCACCACCAATGCACAACAC | ACAGCTCCTATTTTCTGGGCT | 60.27 | 59.08 | 210 | 128 |
100.00%
|
50.00%
|
| Primer 60 | Methanosphaera | GGAGATGATGGACTAGGACCA | TCCCCAGGTGTTGTGTCAAAT | 58.04 | 59.78 | 197 | 128 |
100.00%
|
50.00%
|