| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Methanosphaera | GCAGTAGCATTCATGGATCCA | TGTTAGCCCATGGTCCACG | 58.15 | 59.70 | 166 | 132 |
100.00%
|
33.33%
|
| Primer 32 | Methanosphaera | GCAGTAGCATTCATGGATCCA | ATGTTAGCCCATGGTCCACG | 58.15 | 60.11 | 167 | 132 |
100.00%
|
33.33%
|
| Primer 33 | Methanosphaera | ACCTGCAGAAATTGTAGCACA | TTGGCCTTCACCAGCATCA | 58.14 | 59.54 | 151 | 132 |
100.00%
|
66.67%
|
| Primer 34 | Methanosphaera | AGTCTTGATCCTACGCCATCA | TCTGAACCTTTGCCAAAGCC | 58.61 | 58.96 | 202 | 132 |
100.00%
|
66.67%
|
| Primer 35 | Methanosphaera | AGTCTTGATCCTACGCCATCA | TTCTGAACCTTTGCCAAAGCC | 58.61 | 59.59 | 203 | 132 |
100.00%
|
66.67%
|
| Primer 36 | Methanosphaera | AGTCTTGATCCTACGCCATCA | TCTCCTGACTTTTGTGCTGC | 58.61 | 58.40 | 176 | 132 |
100.00%
|
66.67%
|
| Primer 37 | Methanosphaera | AGTCTTGATCCTACGCCATCA | CCTGACTTTTGTGCTGCTTGA | 58.61 | 59.32 | 173 | 132 |
100.00%
|
66.67%
|
| Primer 38 | Methanosphaera | CCTTGGAGAAAGAAGTCCACG | GAAAATACGAGGGTCGCGAC | 58.58 | 59.08 | 169 | 132 |
100.00%
|
66.67%
|
| Primer 39 | Methanosphaera | TGTCTTCCACGTGTTGGTGT | ACACCAGCTTGAACCGGAT | 59.75 | 59.24 | 176 | 130 |
100.00%
|
33.33%
|
| Primer 40 | Methanosphaera | GGACTTTCAAGCGTTGCGG | GGAGTATCACCTGTTGTGTCC | 60.08 | 58.03 | 251 | 130 |
100.00%
|
33.33%
|