| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Methanosphaera | GCATGGGCACAAGTTGATGT | TTCAGCAACACCCTGTGC | 59.40 | 58.16 | 156 | 137 |
100.00%
|
50.00%
|
| Primer 12 | Methanosphaera | ACCTGCAGAAATTGTAGCACA | TCTTGGCCTTCACCAGCA | 58.14 | 58.75 | 153 | 137 |
100.00%
|
100.00%
|
| Primer 13 | Methanosphaera | ACCTGCAGAAATTGTAGCACA | TTCTTGGCCTTCACCAGCA | 58.14 | 59.46 | 154 | 137 |
100.00%
|
100.00%
|
| Primer 14 | Methanosphaera | ACCTGCAGAAATTGTAGCACA | TCTTGGCCTTCACCAGCAT | 58.14 | 59.23 | 153 | 137 |
100.00%
|
100.00%
|
| Primer 15 | Methanosphaera | ACCTGCAGAAATTGTAGCACA | GTTCTTGGCCTTCACCAGC | 58.14 | 59.04 | 155 | 137 |
100.00%
|
100.00%
|
| Primer 16 | Methanosphaera | ACCTGCAGAAATTGTAGCACA | TCGACGAGTTCTTGGCCTTC | 58.14 | 60.04 | 162 | 137 |
100.00%
|
100.00%
|
| Primer 17 | Methanosphaera | GCAGCAGCAGTAGCATCAC | ACACGGCTTAGTGGTGGTTC | 59.28 | 60.25 | 176 | 136 |
100.00%
|
50.00%
|
| Primer 18 | Methanosphaera | GCAGCAGCAGTAGCATCAC | CACGGCTTAGTGGTGGTTC | 59.28 | 58.46 | 175 | 136 |
100.00%
|
50.00%
|
| Primer 19 | Methanosphaera | ACGTAACCACCCCAAGCAG | CCTCCATCATCTGCAAGTGC | 59.93 | 58.98 | 181 | 135 |
100.00%
|
50.00%
|
| Primer 20 | Methanosphaera | AACCACCCCAAGCAGTAGC | CCTCCATCATCTGCAAGTGC | 59.93 | 58.98 | 177 | 135 |
100.00%
|
50.00%
|