| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Methanosphaera | GGAGATGATGGACTAGGACCA | TCCCCAGGTGTTGTGTCAA | 58.04 | 58.69 | 197 | 130 |
100.00%
|
50.00%
|
| Primer 42 | Methanosphaera | GGAGATGATGGACTAGGACCA | TTCCCCAGGTGTTGTGTCAA | 58.04 | 59.37 | 198 | 130 |
100.00%
|
50.00%
|
| Primer 43 | Methanosphaera | GGAGATGATGGACTAGGACCA | TTTCCCCAGGTGTTGTGTCAA | 58.04 | 59.99 | 199 | 130 |
100.00%
|
50.00%
|
| Primer 44 | Methanosphaera | TCTTGCAGGAGATCAGCCAA | GGGCATACCTAAACCTTCAGC | 59.01 | 58.70 | 151 | 130 |
100.00%
|
100.00%
|
| Primer 45 | Methanosphaera | TGTCTTCCACGTGTTGGTGT | CACACCAGCTTGAACCGGA | 59.75 | 60.23 | 177 | 129 |
100.00%
|
50.00%
|
| Primer 46 | Methanosphaera | TGTCTTCCACGTGTTGGTGT | ACACCAGCTTGAACCGGA | 59.75 | 58.76 | 176 | 129 |
100.00%
|
50.00%
|
| Primer 47 | Methanosphaera | TGGCTGTGATCCTAAGGCTG | ACACCCCTACCAGCACAAC | 59.46 | 59.55 | 186 | 129 |
100.00%
|
100.00%
|
| Primer 48 | Methanosphaera | GGCTGTAAAAGGTCATGGAGC | TCGTATGTTCCATCGGCAAC | 59.25 | 58.35 | 204 | 129 |
100.00%
|
100.00%
|
| Primer 49 | Methanosphaera | CCATGTCCTCCAACAGTGCT | ACGTCTTGCAGCCCAACA | 59.96 | 59.81 | 282 | 128 |
100.00%
|
50.00%
|
| Primer 50 | Methanosphaera | CACCAATGCACAACACAGCA | ACAGCTCCTATTTTCTGGGCT | 59.90 | 59.08 | 205 | 128 |
100.00%
|
50.00%
|