| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Methanosphaera | AGACGTAACCACCCCAAGC | CCTCCATCATCTGCAAGTGC | 59.63 | 58.98 | 183 | 135 |
100.00%
|
33.33%
|
| Primer 22 | Methanosphaera | GGAGATGATGGACTAGGACCA | TCCCCAGGTGTTGTGTCAAA | 58.04 | 59.37 | 197 | 135 |
100.00%
|
33.33%
|
| Primer 23 | Methanosphaera | GGAGATGATGGACTAGGACCA | TTCCCCAGGTGTTGTGTCAAA | 58.04 | 59.99 | 198 | 135 |
100.00%
|
33.33%
|
| Primer 24 | Methanosphaera | GCAGTAGCATTCATGGATCCA | TTAGCCCATGGTCCACGAG | 58.15 | 59.10 | 164 | 135 |
100.00%
|
33.33%
|
| Primer 25 | Methanosphaera | GCAGTAGCATTCATGGATCCA | GTTAGCCCATGGTCCACGAG | 58.15 | 60.46 | 165 | 135 |
100.00%
|
33.33%
|
| Primer 26 | Methanosphaera | TCTTGCAGGAGATCAGCCA | GGGCATACCTAAACCTTCAGC | 58.31 | 58.70 | 151 | 135 |
100.00%
|
66.67%
|
| Primer 27 | Methanosphaera | ATCTTGCAGGAGATCAGCCA | GGGCATACCTAAACCTTCAGC | 58.79 | 58.70 | 152 | 135 |
100.00%
|
66.67%
|
| Primer 28 | Methanosphaera | GGAGATGATGGACTAGGACCA | TTCCCCAGGTGTTGTGTCA | 58.04 | 58.69 | 198 | 132 |
100.00%
|
33.33%
|
| Primer 29 | Methanosphaera | GGAGATGATGGACTAGGACCA | TTTCCCCAGGTGTTGTGTCA | 58.04 | 59.37 | 199 | 132 |
100.00%
|
33.33%
|
| Primer 30 | Methanosphaera | GGAGATGATGGACTAGGACCA | ATTTCCCCAGGTGTTGTGTCA | 58.04 | 59.78 | 200 | 132 |
100.00%
|
33.33%
|