| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Methylorubrum | TTCGAGCCGAACGAAGTCC | AATTGCAGCGTCGCATCC | 60.08 | 59.21 | 194 | 146 |
100.00%
|
50.00%
|
| Primer 2 | Methylorubrum | GCGCTACAACGTCTTGCAG | TGCTGCGCATGTTGTTGG | 59.87 | 59.66 | 245 | 145 |
100.00%
|
100.00%
|
| Primer 3 | Methylorubrum | AAGCTCTCGAACGTCCTGC | TTGACGTAGGTGCGGATCC | 60.08 | 59.49 | 202 | 143 |
100.00%
|
100.00%
|
| Primer 4 | Methylorubrum | TTCCGCAACGAGCTGCAT | TGGTCATGCCGATCACGTC | 60.36 | 60.15 | 286 | 131 |
100.00%
|
100.00%
|
| Primer 5 | Methylorubrum | GCTCTTGCAGCCGAAGTTC | ACATGACGTGCACGGTGT | 59.50 | 59.89 | 179 | 130 |
100.00%
|
100.00%
|
| Primer 6 | Methylorubrum | GAATCGTCGTGCTGCACTG | ACTCCGTCAGGCCATTTCC | 59.58 | 59.70 | 201 | 127 |
100.00%
|
50.00%
|
| Primer 7 | Methylorubrum | TTACCTGTGCGACCACACC | AGGAGACGAAGGCGATCAG | 59.93 | 58.89 | 290 | 126 |
100.00%
|
100.00%
|
| Primer 8 | Methylorubrum | TTACCTGTGCGACCACACC | AAGGAGACGAAGGCGATCAG | 59.93 | 59.54 | 291 | 126 |
100.00%
|
100.00%
|
| Primer 9 | Methylorubrum | TTCCGCAACGAGCTGCAT | TGTAGTGCTTCGACTCGGC | 60.36 | 59.79 | 253 | 125 |
100.00%
|
100.00%
|
| Primer 10 | Methylorubrum | TCGGAGTTGGAGACGAAGC | CGGTAGTCGCGTATCATGC | 59.41 | 58.58 | 185 | 123 |
100.00%
|
50.00%
|