| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Methylorubrum | GCTGGCGATCAACAAGCAG | TGCCGTTGCCGAAGAACA | 59.86 | 60.20 | 293 | 119 |
100.00%
|
75.00%
|
| Primer 22 | Methylorubrum | ATCTCGGTGCTGATGCTGG | TGCCGTTGCCGAAGAACA | 59.85 | 60.20 | 235 | 119 |
100.00%
|
75.00%
|
| Primer 23 | Methylorubrum | TGCTGGAGCGCATCGAAA | ATCCAATGGTCGAGGGCAC | 60.05 | 59.78 | 214 | 119 |
100.00%
|
25.00%
|
| Primer 24 | Methylorubrum | TGCTGGAGCGCATCGAAA | TGAGCAGCATCTCCTCGTG | 60.05 | 59.49 | 177 | 119 |
100.00%
|
25.00%
|
| Primer 25 | Methylorubrum | TGCTGGAGCGCATCGAAA | GATCCAATGGTCGAGGGCA | 60.05 | 59.47 | 215 | 119 |
100.00%
|
25.00%
|
| Primer 26 | Methylorubrum | TGCTGGAGCGCATCGAAA | ATCCAATGGTCGAGGGCA | 60.05 | 58.26 | 214 | 119 |
100.00%
|
25.00%
|
| Primer 27 | Methylorubrum | TGCTGGAGCGCATCGAAA | GCAGGCTCTTGAGCAGCAT | 60.05 | 60.75 | 186 | 119 |
100.00%
|
25.00%
|
| Primer 28 | Methylorubrum | CCTTACGTCCTGTGCCACA | CGTAGCCCTGCATGGTGAT | 59.63 | 59.85 | 154 | 119 |
100.00%
|
50.00%
|
| Primer 29 | Methylorubrum | AAGGGCTTCTGGATCGTGC | AGGTCGGAATTCCAGCAGG | 60.08 | 59.40 | 212 | 118 |
100.00%
|
50.00%
|
| Primer 30 | Methylorubrum | AAGCTCTCGAACGTCCTGC | ACGTAGGTGCGGATCCAGT | 60.08 | 60.68 | 199 | 118 |
100.00%
|
50.00%
|