| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Methylorubrum | AGCGCTACAACGTCTTGC | TGCTGCGCATGTTGTTGG | 58.45 | 59.66 | 246 | 123 |
100.00%
|
25.00%
|
| Primer 12 | Methylorubrum | TGGTGCTCCACTGCTATGC | TCACGCCAGGGTGAATTGG | 60.08 | 60.30 | 263 | 123 |
100.00%
|
25.00%
|
| Primer 13 | Methylorubrum | AAGCTCTCGAACGTCCTGC | TTGACGTAGGTGCGGATCC | 60.08 | 59.49 | 202 | 123 |
100.00%
|
50.00%
|
| Primer 14 | Methylorubrum | AAGCTCTCGAACGTCCTGC | TGATGATCCAGGGCTGCTG | 60.08 | 59.47 | 153 | 123 |
100.00%
|
50.00%
|
| Primer 15 | Methylorubrum | AAGAACGAAGCCCTCCTGC | TGGCCTTGAGGTCCTTGTC | 60.00 | 59.24 | 181 | 122 |
100.00%
|
50.00%
|
| Primer 16 | Methylorubrum | ATGCTGCGCAAGGACGAT | TGGCGAAGACCTTCTGCA | 60.12 | 58.85 | 175 | 120 |
100.00%
|
25.00%
|
| Primer 17 | Methylorubrum | ATGCTGCGCAAGGACGAT | TTGGCGAAGACCTTCTGCA | 60.12 | 59.55 | 176 | 120 |
100.00%
|
25.00%
|
| Primer 18 | Methylorubrum | CACGCTGGCGATCAACAAG | TGCCGTTGCCGAAGAACA | 59.87 | 60.20 | 296 | 119 |
100.00%
|
75.00%
|
| Primer 19 | Methylorubrum | GGCGATCAACAAGCAGCTG | TGCCGTTGCCGAAGAACA | 59.86 | 60.20 | 290 | 119 |
100.00%
|
75.00%
|
| Primer 20 | Methylorubrum | CTGGCGATCAACAAGCAGC | TGCCGTTGCCGAAGAACA | 59.86 | 60.20 | 292 | 119 |
100.00%
|
75.00%
|