| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Ralstonia | GCGACACCGTGAAGAAAGC | TTGACGCTTTTGCCGTGC | 59.80 | 59.97 | 277 | 124 |
100.00%
|
16.67%
|
| Primer 52 | Ralstonia | ACGCAACGCAATCTTCCG | GCGTGAATACCATGTGCCG | 59.14 | 59.65 | 211 | 124 |
100.00%
|
16.67%
|
| Primer 53 | Ralstonia | TGCGCAATCACGGCTACA | TACAGAATGCCCTGCTGCG | 60.05 | 60.45 | 289 | 124 |
100.00%
|
16.67%
|
| Primer 54 | Ralstonia | ATGGCACGCGTCTGTCAA | AGCACTTCGTCGATGCCT | 59.97 | 59.03 | 203 | 122 |
100.00%
|
33.33%
|
| Primer 55 | Ralstonia | ACGGCTGCTGATTGCGTA | TCTTCGGTGATCCACGTGC | 59.74 | 60.08 | 258 | 122 |
100.00%
|
16.67%
|
| Primer 56 | Ralstonia | ACGGCTGCTGATTGCGTA | TCGATGCGGATTTCCCAGG | 59.74 | 59.85 | 180 | 122 |
100.00%
|
16.67%
|
| Primer 57 | Ralstonia | ACGGCTGCTGATTGCGTA | GGTCGATGCGGATTTCCCA | 59.74 | 60.15 | 182 | 122 |
100.00%
|
16.67%
|
| Primer 58 | Ralstonia | ACGGCTGCTGATTGCGTA | TGGTCGATGCGGATTTCCC | 59.74 | 60.15 | 183 | 122 |
100.00%
|
16.67%
|
| Primer 59 | Ralstonia | ACGGCTGCTGATTGCGTA | ACGCCTGGATGACGTCTTC | 59.74 | 59.78 | 272 | 122 |
100.00%
|
16.67%
|
| Primer 60 | Ralstonia | TGCTGGCGTTCCTGTTCA | TCGTGTACGCCATCGCAT | 59.49 | 59.51 | 192 | 121 |
100.00%
|
16.67%
|