| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Ralstonia | CGTTCCGAGACGACTGCAA | ATCGCTTCGGCATACGCT | 60.37 | 59.58 | 193 | 128 |
100.00%
|
16.67%
|
| Primer 32 | Ralstonia | TTTGCGCTGATTGCCGTG | AAACCCAGGGCAAACGCT | 59.75 | 60.12 | 221 | 128 |
100.00%
|
33.33%
|
| Primer 33 | Ralstonia | TGTTTGCGCTGATTGCCG | AAACCCAGGGCAAACGCT | 59.75 | 60.12 | 223 | 128 |
100.00%
|
33.33%
|
| Primer 34 | Ralstonia | TGATTGCCGTGTGCGTGT | AAACCCAGGGCAAACGCT | 60.59 | 60.12 | 214 | 128 |
100.00%
|
33.33%
|
| Primer 35 | Ralstonia | CGTGGTGATGTTTGCGCT | AAACCCAGGGCAAACGCT | 59.05 | 60.12 | 231 | 128 |
100.00%
|
33.33%
|
| Primer 36 | Ralstonia | TTGCGCTGATTGCCGTGT | AAACCCAGGGCAAACGCT | 60.98 | 60.12 | 220 | 128 |
100.00%
|
33.33%
|
| Primer 37 | Ralstonia | CCTGGTTGTCAGTGGGCAT | AAACCCAGGGCAAACGCT | 59.92 | 60.12 | 160 | 128 |
100.00%
|
33.33%
|
| Primer 38 | Ralstonia | ACGACGCTGTTCTACCTGC | AAACCCAGGGCAAACGCT | 60.08 | 60.12 | 269 | 128 |
100.00%
|
33.33%
|
| Primer 39 | Ralstonia | GTGCCATGTTTGCTCTGCC | TGCCGTCGTTGTTGGTGT | 60.08 | 60.13 | 294 | 127 |
100.00%
|
16.67%
|
| Primer 40 | Ralstonia | TGCCATGTTTGCTCTGCC | TGCCGTCGTTGTTGGTGT | 58.63 | 60.13 | 293 | 127 |
100.00%
|
16.67%
|