| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Ralstonia | TCAGTCCGATCCGCACAAG | TGCGTGCAAGCAGGAGAT | 59.78 | 59.65 | 188 | 129 |
100.00%
|
50.00%
|
| Primer 22 | Ralstonia | CAAGCAAATGGGGTTGGCC | TGCGCATGCCTTCCGAAT | 60.00 | 60.44 | 212 | 128 |
100.00%
|
50.00%
|
| Primer 23 | Ralstonia | CGAATTTGCCGTGCTGGAG | CGCGTGCCTGCAACAAAT | 59.86 | 60.05 | 230 | 128 |
100.00%
|
50.00%
|
| Primer 24 | Ralstonia | CGAATTTGCCGTGCTGGA | CGCGTGCCTGCAACAAAT | 58.74 | 60.05 | 230 | 128 |
100.00%
|
50.00%
|
| Primer 25 | Ralstonia | CCGTGCTGGAGTTGCTGAT | CGCGTGCCTGCAACAAAT | 60.38 | 60.05 | 222 | 128 |
100.00%
|
50.00%
|
| Primer 26 | Ralstonia | TGCTGGAGTTGCTGATGCT | CGCGTGCCTGCAACAAAT | 59.62 | 60.05 | 219 | 128 |
100.00%
|
50.00%
|
| Primer 27 | Ralstonia | TTTGCCGTGCTGGAGTTG | CGCGTGCCTGCAACAAAT | 58.57 | 60.05 | 226 | 128 |
100.00%
|
50.00%
|
| Primer 28 | Ralstonia | GTGCTGGAGTTGCTGATGC | CGCGTGCCTGCAACAAAT | 59.49 | 60.05 | 220 | 128 |
100.00%
|
50.00%
|
| Primer 29 | Ralstonia | AGATCGTTGCCTTCGCCA | TGTTGACCGACACGTGCT | 59.34 | 59.50 | 264 | 128 |
100.00%
|
50.00%
|
| Primer 30 | Ralstonia | AAGAGCTCGCCTTTCGCA | TACGTTGACTGGCACGCT | 59.66 | 59.27 | 269 | 128 |
100.00%
|
50.00%
|