| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Ralstonia | GCATGTTCCTGCGCAACT | TCAGCACGTCATCGCACA | 59.04 | 59.66 | 237 | 126 |
100.00%
|
16.67%
|
| Primer 42 | Ralstonia | GCGCAACTACGAAGTGCAG | TCAGCACGTCATCGCACA | 59.87 | 59.66 | 227 | 126 |
100.00%
|
16.67%
|
| Primer 43 | Ralstonia | CTGCGCAACTACGAAGTGC | TCAGCACGTCATCGCACA | 59.87 | 59.66 | 229 | 126 |
100.00%
|
16.67%
|
| Primer 44 | Ralstonia | CATCAGTCCGATCCGCACA | TGCGTGCAAGCAGGAGAT | 59.86 | 59.65 | 190 | 126 |
100.00%
|
16.67%
|
| Primer 45 | Ralstonia | ACAACACGCCATGATCGC | GGCACGTACTTCCACGACA | 58.82 | 60.01 | 210 | 125 |
100.00%
|
50.00%
|
| Primer 46 | Ralstonia | TGGCCTCACACGGTTTGA | TGAACAGCAGGATCACCGC | 59.09 | 60.38 | 228 | 125 |
100.00%
|
33.33%
|
| Primer 47 | Ralstonia | ATCCTGCGCACGAAGAAGG | CGTGAACACCTTGTCGTGC | 60.45 | 59.72 | 156 | 124 |
100.00%
|
16.67%
|
| Primer 48 | Ralstonia | CATTGCCGTGAACGTGGTG | ACGCTGCGCATTGATCCA | 60.08 | 60.44 | 193 | 124 |
100.00%
|
16.67%
|
| Primer 49 | Ralstonia | ATTGCCGTGAACGTGGTG | ACGCTGCGCATTGATCCA | 58.66 | 60.44 | 192 | 124 |
100.00%
|
16.67%
|
| Primer 50 | Ralstonia | TCGATGGATGCGCTGCAA | AGCATCAGTCCGATCAGCG | 60.13 | 59.93 | 200 | 124 |
100.00%
|
16.67%
|