| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Ralstonia | TGCGCAATCACGGCTACA | CATGACAAACGTGCTGCCC | 60.05 | 60.08 | 221 | 137 |
100.00%
|
16.67%
|
| Primer 12 | Ralstonia | TGCGCAATCACGGCTACA | ATGACAAACGTGCTGCCC | 60.05 | 58.65 | 220 | 137 |
100.00%
|
16.67%
|
| Primer 13 | Ralstonia | TGCGCAATCACGGCTACA | CAGCGCAATCGACCAGTTC | 60.05 | 59.58 | 257 | 137 |
100.00%
|
16.67%
|
| Primer 14 | Ralstonia | GCATCCCCAATTGCGACAC | TTGACGCTTTTGCCGTGC | 59.86 | 59.97 | 289 | 135 |
100.00%
|
16.67%
|
| Primer 15 | Ralstonia | CACAACACGATGCAGGCAC | TTGCCGTGCATGGGTTGA | 60.08 | 60.20 | 263 | 132 |
100.00%
|
33.33%
|
| Primer 16 | Ralstonia | GTGCTGCTGCTTAACGACG | TTGCCGTGCATGGGTTGA | 59.87 | 60.20 | 195 | 132 |
100.00%
|
16.67%
|
| Primer 17 | Ralstonia | AGCGACCACTCCACAACA | TTGCCGTGCATGGGTTGA | 58.77 | 60.20 | 274 | 132 |
100.00%
|
33.33%
|
| Primer 18 | Ralstonia | AGCGACCACTCCACAACAC | TTGCCGTGCATGGGTTGA | 60.23 | 60.20 | 274 | 132 |
100.00%
|
33.33%
|
| Primer 19 | Ralstonia | ATCGTTGCTGCCCTGCAT | TTCTTCCGACACCACCACG | 60.04 | 59.93 | 270 | 132 |
100.00%
|
33.33%
|
| Primer 20 | Ralstonia | CCTGGAGCGGAAAGAGCAA | TTGCCGTGCATGGGTTGA | 60.00 | 60.20 | 241 | 130 |
100.00%
|
33.33%
|