| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1221 | Thermotoga | AGGCAGCGGTTGAGGTTT | TCGGGATGAGAAGCGGAGT | 59.48 | 60.38 | 265 | 87 |
100.00%
|
60.00%
|
| Primer 1222 | Thermotoga | ACGATCATTGTGGGAGTGGC | GCTGGGCAAAGCAACATCC | 60.39 | 60.08 | 240 | 87 |
100.00%
|
60.00%
|
| Primer 1223 | Thermotoga | TTCCGAACTGCAAGCGGT | ACGAACACGCCTCGCTTT | 59.89 | 60.28 | 156 | 87 |
100.00%
|
40.00%
|
| Primer 1224 | Thermotoga | TCCGTTGAGGTGCAGATCG | ACTTGGCGATGAGTTCTCGA | 59.78 | 59.11 | 184 | 87 |
100.00%
|
80.00%
|
| Primer 1225 | Thermotoga | CCCTCGCGAACGTCATCAT | TCAGCGGTTTCCTCGAGT | 60.23 | 58.23 | 250 | 87 |
100.00%
|
80.00%
|
| Primer 1226 | Thermotoga | TCGCGAGGGCTTTGTTGA | AGCCTGCGCTCTGTCTTT | 59.58 | 59.25 | 197 | 87 |
100.00%
|
80.00%
|
| Primer 1227 | Thermotoga | GGGGTGGAAGAGCCTGTTT | CGAAGAACCACTCACCGCT | 59.54 | 60.01 | 152 | 87 |
100.00%
|
100.00%
|
| Primer 1228 | Thermotoga | GGGGTGGAAGAGCCTGTTT | TCGAAGAACCACTCACCGC | 59.54 | 60.01 | 153 | 87 |
100.00%
|
100.00%
|
| Primer 1229 | Thermotoga | TGAAGTGTGCGGAAAGGC | ACACCTCCGAAACCTGACC | 58.26 | 59.25 | 197 | 87 |
100.00%
|
100.00%
|
| Primer 1230 | Thermotoga | TGCTATCCTGTCCATGTGGC | TGAAAGTCGACACGCCTGT | 59.82 | 59.56 | 161 | 87 |
100.00%
|
60.00%
|