| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1231 | Thermotoga | ACGATCATTGTGGGAGTGGC | GCTGGGCAAAGCAACATCC | 60.39 | 60.08 | 240 | 87 |
100.00%
|
60.00%
|
| Primer 1232 | Thermotoga | GGAACGACTCAGCCGAACA | TCCTTTCTCGCACGTTCGA | 60.01 | 59.34 | 161 | 87 |
100.00%
|
80.00%
|
| Primer 1233 | Thermotoga | GTGATCTTCGGCTCTGCGA | TCAGCGTGGCAAAGACGT | 59.86 | 59.89 | 286 | 87 |
100.00%
|
80.00%
|
| Primer 1234 | Thermotoga | CTTTGCTTGAAGCCCTCGC | CCCAGAACAGCCAGGGTTT | 59.79 | 59.85 | 213 | 87 |
100.00%
|
80.00%
|
| Primer 1235 | Thermotoga | GGGGTGGAAGAGCCTGTTT | CGAAGAACCACTCACCGCT | 59.54 | 60.01 | 152 | 87 |
100.00%
|
100.00%
|
| Primer 1236 | Thermotoga | GGGGTGGAAGAGCCTGTTT | TCGAAGAACCACTCACCGC | 59.54 | 60.01 | 153 | 87 |
100.00%
|
100.00%
|
| Primer 1237 | Thermotoga | GGAATCTGTGCCTACGCGA | AACAACCATCGGCAGCCA | 59.86 | 59.88 | 210 | 87 |
100.00%
|
80.00%
|
| Primer 1238 | Thermotoga | GGTTGGACTCAGCGTCACA | ACACGCTCGCCAGCTTTT | 59.93 | 60.59 | 176 | 87 |
100.00%
|
80.00%
|
| Primer 1239 | Thermotoga | TCGTGGTTGGTGGGCTTT | ACTCGTGAACCAACCCAGG | 59.40 | 59.55 | 187 | 87 |
100.00%
|
40.00%
|
| Primer 1240 | Thermotoga | TGCTCGTACTGACAGTGGC | TGAGACCGTAGCCGAAACC | 59.71 | 59.41 | 198 | 87 |
100.00%
|
80.00%
|