| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1241 | Thermotoga | TGCTCGTACTGACAGTGGC | AGACCGTAGCCGAAACCTC | 59.71 | 59.11 | 196 | 87 |
100.00%
|
80.00%
|
| Primer 1242 | Thermotoga | TGAACACGGGAGCAACGT | TCTCTTCCCTCGACCACGA | 59.50 | 59.62 | 286 | 87 |
100.00%
|
80.00%
|
| Primer 1243 | Thermotoga | CCCTCGCGAACGTCATCAT | TCAGCGGTTTCCTCGAGT | 60.23 | 58.23 | 250 | 87 |
100.00%
|
80.00%
|
| Primer 1244 | Thermotoga | TCCGTTGAGGTGCAGATCG | ACTTGGCGATGAGTTCTCGA | 59.78 | 59.11 | 184 | 87 |
100.00%
|
80.00%
|
| Primer 1245 | Thermotoga | TCCGATGTGGCGTTTCGA | CACGGTTTCTCCAACGGGA | 59.35 | 59.93 | 210 | 87 |
100.00%
|
60.00%
|
| Primer 1246 | Thermotoga | ATCGAGCCACAACAGACGA | CCGCTTCTTCGGGATGAGA | 59.04 | 59.18 | 237 | 87 |
100.00%
|
80.00%
|
| Primer 1247 | Thermotoga | GATCGAGCCACAACAGACGA | CCGCTTCTTCGGGATGAGA | 60.11 | 59.18 | 238 | 87 |
100.00%
|
80.00%
|
| Primer 1248 | Thermotoga | AGGAACAACTCAGCCGAACA | TCCTTTCTCGCACGTTCGA | 59.53 | 59.34 | 162 | 87 |
100.00%
|
80.00%
|
| Primer 1249 | Thermotoga | CAGCCGAACATTTCACAGCA | TCCTTTCTCGCACGTTCGA | 59.41 | 59.34 | 152 | 87 |
100.00%
|
80.00%
|
| Primer 1250 | Thermotoga | CCCGATGGAGCCAAGAAAGT | ACGGTCTGTCAGCTCCTGT | 60.04 | 60.53 | 271 | 87 |
100.00%
|
40.00%
|