| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1261 | Thermotoga | TATGCCGTACCACACAGGC | TCACACCGAGCACTGCAA | 59.78 | 59.50 | 188 | 86 |
100.00%
|
100.00%
|
| Primer 1262 | Thermotoga | AGAACGAGGTTCAGAGCGG | TGTCGCACTTGTGAGCCA | 59.41 | 59.50 | 181 | 86 |
100.00%
|
80.00%
|
| Primer 1263 | Thermotoga | ACAACCCCACAAGTTACCGG | TCCAGCAGATCCAGCACA | 60.18 | 58.19 | 270 | 86 |
100.00%
|
80.00%
|
| Primer 1264 | Thermotoga | CCCGTTGGAAGGCTCGATA | ACGTGTCGTTCGACAGCT | 59.18 | 59.28 | 229 | 86 |
100.00%
|
80.00%
|
| Primer 1265 | Thermotoga | ATTCACGGTCCGCTGCAT | AGCTTCCGGTGTGCCATA | 59.73 | 58.60 | 168 | 86 |
100.00%
|
40.00%
|
| Primer 1266 | Thermotoga | GGCCACCAAAGCCGACATA | ACCTTCCCCTCGAGAGCAA | 60.38 | 60.23 | 198 | 86 |
100.00%
|
80.00%
|
| Primer 1267 | Thermotoga | ACTCCGCTGTTTACAGGCC | TGGAGCCACCAACCACAA | 60.00 | 58.99 | 186 | 86 |
100.00%
|
40.00%
|
| Primer 1268 | Thermotoga | CGCTGTTTACAGGCCTGGA | TGGAGCCACCAACCACAA | 60.00 | 58.99 | 182 | 86 |
100.00%
|
40.00%
|
| Primer 1269 | Thermotoga | TCCGCTGTTTACAGGCCTG | TGGAGCCACCAACCACAA | 60.00 | 58.99 | 184 | 86 |
100.00%
|
40.00%
|
| Primer 1270 | Thermotoga | CCGTTTGACGCTGTTCTGG | CACTGGTTCCCGTCGTCAA | 59.43 | 59.93 | 151 | 86 |
100.00%
|
80.00%
|