| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1281 | Thermotoga | AACGCCATGGCACTTGGA | AGGTTCTTGCTGCCGTGT | 59.88 | 59.49 | 256 | 84 |
100.00%
|
80.00%
|
| Primer 1282 | Thermotoga | CGGAAGATGTTCGAGCCCA | ACGCAATCCACGTGAGGT | 59.78 | 59.26 | 161 | 84 |
100.00%
|
80.00%
|
| Primer 1283 | Thermotoga | TGGTCTGTCCGCAGCAAA | TGTGTGTGCCCACCTGAA | 59.49 | 59.00 | 153 | 84 |
100.00%
|
80.00%
|
| Primer 1284 | Thermotoga | TGAGGCGTCCGTTTTGCT | GTGAAGTTGCTCCCACGGA | 59.89 | 59.93 | 212 | 84 |
100.00%
|
40.00%
|
| Primer 1285 | Thermotoga | TAGCCCTATCACCAGCGGT | ACGAGCGCAGGGTTGAAA | 60.08 | 59.89 | 298 | 84 |
100.00%
|
60.00%
|
| Primer 1286 | Thermotoga | TGGCTACGAACTCTCCGGA | AGCAGCAAGGAAGGTCACC | 60.00 | 59.93 | 286 | 84 |
100.00%
|
100.00%
|
| Primer 1287 | Thermotoga | CGGTGTTGAAGCACACGTG | GATCGAGCCCTGCACAGAA | 60.01 | 59.78 | 170 | 84 |
100.00%
|
80.00%
|
| Primer 1288 | Thermotoga | GAAGGCAACCAACCGAGGA | TGTCGAGAAGGTTGCCGAG | 59.93 | 59.71 | 182 | 84 |
100.00%
|
80.00%
|
| Primer 1289 | Thermotoga | TTTCGTTACCCCAAGCGGT | TCGTGTTCCCGTTGCTGT | 59.55 | 59.50 | 155 | 84 |
100.00%
|
40.00%
|
| Primer 1290 | Thermotoga | TGCAAGCGATGTGACGGT | TGAAGTTCGCAGAGCCGAA | 59.97 | 59.63 | 182 | 84 |
100.00%
|
40.00%
|