| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1271 | Thermotoga | TTGGAAGTGCTGCGCTGA | GCGTTCGCTTCTTTTCCGG | 59.89 | 60.15 | 157 | 86 |
100.00%
|
80.00%
|
| Primer 1272 | Thermotoga | GGCTTGAACATGTGTGCGG | TTCCGACAACCGTGGCAA | 60.08 | 59.81 | 156 | 86 |
100.00%
|
80.00%
|
| Primer 1273 | Thermotoga | TGGCCATGTTGCGTCCAT | TTTCCCACAAGCCAGACGG | 59.96 | 60.23 | 252 | 86 |
100.00%
|
80.00%
|
| Primer 1274 | Thermotoga | ATGGCCATGTTGCGTCCA | TTTCCCACAAGCCAGACGG | 59.96 | 60.23 | 253 | 86 |
100.00%
|
80.00%
|
| Primer 1275 | Thermotoga | GCTGAACGACTTTGCCACG | TCGGGATGCTCTTTGGCAA | 60.08 | 59.62 | 168 | 86 |
100.00%
|
40.00%
|
| Primer 1276 | Thermotoga | TGATGGTCTTCGAGTGCGG | TCACACCGAGCACTGCAA | 59.78 | 59.50 | 237 | 86 |
100.00%
|
60.00%
|
| Primer 1277 | Thermotoga | TGATGGTCTTCGAGTGCGG | TTCACACCGAGCACTGCA | 59.78 | 59.50 | 238 | 86 |
100.00%
|
60.00%
|
| Primer 1278 | Thermotoga | AATCGAGCCGAACCTGGAC | TCACACCGAGCACTGCAA | 59.78 | 59.50 | 257 | 86 |
100.00%
|
60.00%
|
| Primer 1279 | Thermotoga | CCCTGTGGGCACTTCAGAT | TTGCAACCTCCACCGCAT | 59.31 | 59.88 | 152 | 85 |
100.00%
|
40.00%
|
| Primer 1280 | Thermotoga | ACCGTGGAATGTGGAGCAG | TTCCCCAGCCCTCAAGGAT | 60.00 | 60.23 | 163 | 85 |
100.00%
|
40.00%
|