| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1821 | Thermotoga | GGCCACCAAAGCCGACATA | CTCGAGAGCAAGGCTGGTT | 60.38 | 59.71 | 190 | 75 |
100.00%
|
80.00%
|
| Primer 1822 | Thermotoga | TTGGTCTGTCCGCAGCAA | ATCAGCTGTGTGTGCCCA | 59.49 | 59.16 | 160 | 74 |
100.00%
|
80.00%
|
| Primer 1823 | Thermotoga | AGCGGTGGCGAACTTCAA | TCAGCAAATCGCAGAGCCA | 59.89 | 60.00 | 200 | 74 |
100.00%
|
40.00%
|
| Primer 1824 | Thermotoga | AAGGGGAAGTTGTGGCGAG | TCGAGTTCGCCTTCATCGG | 59.93 | 59.86 | 181 | 74 |
100.00%
|
80.00%
|
| Primer 1825 | Thermotoga | AGTTCGCCTTCACCGCAA | CACTGCTTTCCCCACACCA | 59.89 | 60.15 | 245 | 74 |
100.00%
|
80.00%
|
| Primer 1826 | Thermotoga | AGTTCGCCTTCACCGCAA | CCAGGTCGAACAGGGCAAA | 59.89 | 60.23 | 207 | 74 |
100.00%
|
80.00%
|
| Primer 1827 | Thermotoga | AGTTCGCCTTCACCGCAA | CAACCGATGCTTCTCCCCA | 59.89 | 59.70 | 183 | 74 |
100.00%
|
80.00%
|
| Primer 1828 | Thermotoga | GTTCACTCCTGCGCTGGAT | AGAGCCTTTGCCCATGGAC | 60.08 | 60.00 | 182 | 74 |
100.00%
|
100.00%
|
| Primer 1829 | Thermotoga | GTTCACTCCTGCGCTGGAT | TCAGAGCCTTTGCCCATGG | 60.08 | 60.00 | 184 | 74 |
100.00%
|
100.00%
|
| Primer 1830 | Thermotoga | AACGGTGTGGAAGTGGAGG | CCTTCGGCACCTTCACGAT | 59.55 | 60.08 | 160 | 74 |
100.00%
|
80.00%
|