| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1831 | Thermotoga | AGAGCTCACTCACGCGATG | AACTCACGGTGGCGTGAT | 59.86 | 59.26 | 209 | 74 |
100.00%
|
60.00%
|
| Primer 1832 | Thermotoga | TGGGAATCGGCATCGCAA | TTCGTCGATGACCGTGCA | 59.73 | 59.35 | 152 | 74 |
100.00%
|
40.00%
|
| Primer 1833 | Thermotoga | AAAGGGTTGGTGGTGTCGG | GCTTCCACCGCTTCTCTGT | 60.15 | 60.00 | 161 | 74 |
100.00%
|
80.00%
|
| Primer 1834 | Thermotoga | GCGCTGAAAGAAGCACTGG | CTTCGACGGTGAGCCTCAA | 59.79 | 59.71 | 151 | 74 |
100.00%
|
80.00%
|
| Primer 1835 | Thermotoga | GTCGTTGAAGCCAAAGCGG | CCCACGCGTCTTTCTCGAT | 60.08 | 60.15 | 208 | 74 |
100.00%
|
40.00%
|
| Primer 1836 | Thermotoga | TGGAGAGGGTGCTTCGTGA | TTCCAGCACTGAGAAGCGG | 60.53 | 60.00 | 290 | 74 |
100.00%
|
80.00%
|
| Primer 1837 | Thermotoga | TCGACGGTGTGTTTCTCGG | TCCCTGCGGTTGTGAGAAC | 60.01 | 59.93 | 192 | 74 |
100.00%
|
40.00%
|
| Primer 1838 | Thermotoga | TCGACGGTGTGTTTCTCGG | CCCTGCGGTTGTGAGAACT | 60.01 | 59.93 | 191 | 74 |
100.00%
|
40.00%
|
| Primer 1839 | Thermotoga | GTTCACTCCTGCGCTGGAT | AGAGCCTTTGCCCATGGAC | 60.08 | 60.00 | 182 | 74 |
100.00%
|
100.00%
|
| Primer 1840 | Thermotoga | GTTCACTCCTGCGCTGGAT | TCAGAGCCTTTGCCCATGG | 60.08 | 60.00 | 184 | 74 |
100.00%
|
100.00%
|