| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1841 | Thermotoga | AGAGCTCACTCACGCGATG | AACTCACGGTGGCGTGAT | 59.86 | 59.26 | 209 | 74 |
100.00%
|
60.00%
|
| Primer 1842 | Thermotoga | TCGTTGGGTTCGGAATCGG | AAGAACCCGTCCGCAACA | 60.08 | 59.49 | 268 | 74 |
100.00%
|
80.00%
|
| Primer 1843 | Thermotoga | GTTCACTCCTGCGCTGGAT | AGAGCCTTTGCCCATGGAC | 60.08 | 60.00 | 182 | 74 |
100.00%
|
100.00%
|
| Primer 1844 | Thermotoga | GTTCACTCCTGCGCTGGAT | TCAGAGCCTTTGCCCATGG | 60.08 | 60.00 | 184 | 74 |
100.00%
|
100.00%
|
| Primer 1845 | Thermotoga | TGCCGTTGGACTCGTTGT | TGGAAGGGGAATGATGCGG | 59.50 | 59.77 | 244 | 74 |
100.00%
|
80.00%
|
| Primer 1846 | Thermotoga | TTGGTCTGTCCGCAGCAA | ATCAGCTGTGTGTGCCCA | 59.49 | 59.16 | 160 | 74 |
100.00%
|
80.00%
|
| Primer 1847 | Thermotoga | AAACGTCACACCGGACTCC | TCTTCCTCGTCGACGGGAT | 59.93 | 60.08 | 156 | 74 |
100.00%
|
80.00%
|
| Primer 1848 | Thermotoga | ATTCACGGTCCGCTGCAT | AGCTTCCGGTGTGCCATAA | 59.73 | 59.32 | 168 | 74 |
100.00%
|
40.00%
|
| Primer 1849 | Thermotoga | GAAGAGGGAGCGGACTCATC | CGGTGGTTGGAAGCTCGAT | 59.61 | 60.08 | 294 | 74 |
100.00%
|
80.00%
|
| Primer 1850 | Thermotoga | GGCATACCGTTTGACGCTG | ACTGGTTCCCGTCGTCAA | 59.58 | 58.46 | 156 | 74 |
100.00%
|
80.00%
|