| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1861 | Thermotoga | CGAAACGTACAACGAGGGGA | AAGTCTTCCGTGGCGTGT | 60.04 | 59.18 | 234 | 73 |
100.00%
|
80.00%
|
| Primer 1862 | Thermotoga | GTTCACTCCTGCGCTGGAT | CAGAGCCTTTGCCCATGGA | 60.08 | 60.00 | 183 | 73 |
100.00%
|
100.00%
|
| Primer 1863 | Thermotoga | TCGTTGAAGCCAAAGCGGA | CCCACGCGTCTTTCTCGAT | 60.23 | 60.15 | 207 | 73 |
100.00%
|
40.00%
|
| Primer 1864 | Thermotoga | TAAACGAAGCGCTCCGGA | CGAGCTCGTTCATTTCCCC | 59.04 | 58.62 | 165 | 73 |
100.00%
|
80.00%
|
| Primer 1865 | Thermotoga | TTCCCTTCAGCTCGCAAGG | AAACGGTTCGCCATGGGA | 60.00 | 59.57 | 156 | 73 |
100.00%
|
80.00%
|
| Primer 1866 | Thermotoga | AGCTGAGTGGGTGGAAGGA | TCGCGAACGACAAAGGAGG | 60.15 | 60.37 | 204 | 73 |
100.00%
|
40.00%
|
| Primer 1867 | Thermotoga | AAGGAGCTGAGTGGGTGGA | TCGCGAACGACAAAGGAGG | 60.15 | 60.37 | 208 | 73 |
100.00%
|
40.00%
|
| Primer 1868 | Thermotoga | GTTCACTCCTGCGCTGGAT | CAGAGCCTTTGCCCATGGA | 60.08 | 60.00 | 183 | 73 |
100.00%
|
100.00%
|
| Primer 1869 | Thermotoga | GCCATGGTGTTGGAGTGGA | TCAATCCCTGCGCTCCTTG | 59.93 | 60.08 | 152 | 73 |
100.00%
|
100.00%
|
| Primer 1870 | Thermotoga | CAACCCCACAAGTTACCGGA | GCAATCCAGCAGATCCAGC | 59.89 | 58.97 | 273 | 73 |
100.00%
|
80.00%
|