| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1851 | Thermotoga | CTCAGCTGGCAGCCGATAA | TCTTCTTGGGTCTGCAGCC | 59.86 | 59.62 | 189 | 74 |
100.00%
|
80.00%
|
| Primer 1852 | Thermotoga | TGAGGGTCGTGCGCTTAA | ACCTGCCGATCGGAACAA | 58.95 | 58.93 | 180 | 74 |
100.00%
|
40.00%
|
| Primer 1853 | Thermotoga | TGAGGGTCGTGCGCTTAA | GTTCTTTCCCACCTGCCGA | 58.95 | 59.93 | 190 | 74 |
100.00%
|
40.00%
|
| Primer 1854 | Thermotoga | TGAGGGTCGTGCGCTTAA | CCGATCGGAACAACAACGC | 58.95 | 59.87 | 175 | 74 |
100.00%
|
40.00%
|
| Primer 1855 | Thermotoga | AAAGGGTTGGTGGTGTCGG | GCTTCCACCGCTTCTCTGT | 60.15 | 60.00 | 161 | 74 |
100.00%
|
80.00%
|
| Primer 1856 | Thermotoga | TGTTCGGTGCCATTCCCAA | TGACGGCATCCACGTTGA | 59.85 | 59.26 | 243 | 74 |
100.00%
|
80.00%
|
| Primer 1857 | Thermotoga | ACGCGAACGAGCAGAATCA | CTTCAAACGCGTCGGCAA | 60.08 | 59.37 | 288 | 74 |
100.00%
|
40.00%
|
| Primer 1858 | Thermotoga | AACGTGGTGTTCGAGCCA | TCCTTCCGTGGTTTTCCGG | 59.50 | 59.93 | 285 | 74 |
100.00%
|
100.00%
|
| Primer 1859 | Thermotoga | AACGCCATGGCACTTGGA | AAGGTTCTTGCTGCCGTGT | 59.88 | 60.15 | 257 | 73 |
100.00%
|
80.00%
|
| Primer 1860 | Thermotoga | TTGGACACGTGCTTACCCC | ACTGCACCTCCATGACGAC | 59.93 | 59.71 | 291 | 73 |
100.00%
|
80.00%
|