| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1971 | Thermotoga | TCGACGAAGCAACGGACA | GGCTTCCGCTTCACCTTCT | 59.28 | 60.00 | 224 | 71 |
100.00%
|
80.00%
|
| Primer 1972 | Thermotoga | TTGTGGTGGGTGGGGATCT | TGTTTCCTGCGTGTGTGC | 60.47 | 58.89 | 152 | 71 |
100.00%
|
60.00%
|
| Primer 1973 | Thermotoga | ATCCTGGGAGCGGACATCT | AAGTCACGTGAGCTGGCT | 60.07 | 58.86 | 163 | 71 |
100.00%
|
80.00%
|
| Primer 1974 | Thermotoga | CGATAGATTCGCCAGGGCA | ACGCGTTTCTGCCGACAT | 59.63 | 60.36 | 173 | 71 |
100.00%
|
40.00%
|
| Primer 1975 | Thermotoga | CAGGATCCGTTGAGGTGCA | TCCGGTTTCTTGTGTCTCAGG | 59.70 | 59.93 | 163 | 71 |
100.00%
|
80.00%
|
| Primer 1976 | Thermotoga | TGGTCACAGTCTCGGTGGA | GTACGCTTCCACCGCTTCT | 60.15 | 60.08 | 229 | 71 |
100.00%
|
80.00%
|
| Primer 1977 | Thermotoga | AAAGGGTTGGTGGTGTCGG | GTACGCTTCCACCGCTTCT | 60.15 | 60.08 | 165 | 71 |
100.00%
|
80.00%
|
| Primer 1978 | Thermotoga | AGTGGTTGTTGCCGTGGA | TTGCCCTCGACTGCTTCT | 59.41 | 58.53 | 252 | 71 |
100.00%
|
80.00%
|
| Primer 1979 | Thermotoga | AGTGGTTGTTGCCGTGGAA | CTTGCCCTCGACTGCTTCT | 60.08 | 59.71 | 253 | 71 |
100.00%
|
80.00%
|
| Primer 1980 | Thermotoga | CGGTGGAAAGGTGCAGTCT | CGAGTTTCCCGTTCACCCA | 59.93 | 59.93 | 227 | 71 |
100.00%
|
80.00%
|