| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1981 | Thermotoga | CGGTGGAAAGGTGCAGTCT | ACGAGTTTCCCGTTCACCC | 59.93 | 59.93 | 228 | 71 |
100.00%
|
80.00%
|
| Primer 1982 | Thermotoga | GGTGTCACCGAGTTGAGCA | AGGTCTGCCGTGAGATGTG | 59.93 | 59.41 | 254 | 71 |
100.00%
|
80.00%
|
| Primer 1983 | Thermotoga | ACACATCTGGACCCGAGGA | TTTCCCTCGTCGTTGAGCA | 59.92 | 59.26 | 194 | 71 |
100.00%
|
40.00%
|
| Primer 1984 | Thermotoga | TGGGAAGTGGCGAATCGAG | TCGCCACATCCGTGTTCT | 59.78 | 58.94 | 178 | 71 |
100.00%
|
40.00%
|
| Primer 1985 | Thermotoga | ACCAGGCGTTCGACATCAG | ATGGTTTGCCTGGCCACT | 60.08 | 59.47 | 226 | 71 |
100.00%
|
40.00%
|
| Primer 1986 | Thermotoga | AATGGCAGGAGTTGGGGTC | GTTCGGCAAACGTATGGCC | 59.62 | 59.86 | 172 | 71 |
100.00%
|
40.00%
|
| Primer 1987 | Thermotoga | ATGAGAAAGCCGTGGACACT | TCCCAATGGAGCAATCGGTC | 59.31 | 60.11 | 167 | 71 |
100.00%
|
60.00%
|
| Primer 1988 | Thermotoga | ACGAAAACTGGGACGGCA | CCAGTGCCTCTTCGTGTGT | 59.49 | 59.93 | 159 | 71 |
100.00%
|
80.00%
|
| Primer 1989 | Thermotoga | AGTTCTGGCCAACGGTGT | AGAAGCAGCCTCGTAAGCC | 59.08 | 59.78 | 193 | 71 |
100.00%
|
60.00%
|
| Primer 1990 | Thermotoga | CCAGCTTGTGGTTGACGGA | ACACCGTTGGCCAGAACT | 60.23 | 59.08 | 261 | 71 |
100.00%
|
60.00%
|