| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1991 | Thermotoga | AACTCGACGGAGAAAGGGC | ACACCGTTGGCCAGAACT | 59.71 | 59.08 | 184 | 71 |
100.00%
|
60.00%
|
| Primer 1992 | Thermotoga | TCTGATCACCCGACCGGAT | TTCCTGCGTGCCCATTCT | 60.08 | 59.24 | 258 | 71 |
100.00%
|
60.00%
|
| Primer 1993 | Thermotoga | TTCTGATCACCCGACCGGA | TTCCTGCGTGCCCATTCT | 60.30 | 59.24 | 259 | 71 |
100.00%
|
80.00%
|
| Primer 1994 | Thermotoga | ACCTGGCGTCTTGTGAAGG | ATCAGCGTGTGGGCTTCT | 59.93 | 58.93 | 185 | 71 |
100.00%
|
80.00%
|
| Primer 1995 | Thermotoga | ACCTGGCGTCTTGTGAAGG | GATCAGCGTGTGGGCTTCT | 59.93 | 60.08 | 186 | 71 |
100.00%
|
80.00%
|
| Primer 1996 | Thermotoga | TTTCTCGACGTCACAGGCC | TGCCACTTCCTTCGGATAGC | 60.01 | 59.82 | 234 | 71 |
100.00%
|
40.00%
|
| Primer 1997 | Thermotoga | TTGGAGTGACTGCTGCAGC | TGTTTGGGAACACCGCTCT | 60.60 | 59.47 | 241 | 71 |
100.00%
|
80.00%
|
| Primer 1998 | Thermotoga | TGTGGCGAAACCTGTGGT | GCTCCGCATCCACCTTTCT | 59.41 | 60.08 | 263 | 71 |
100.00%
|
40.00%
|
| Primer 1999 | Thermotoga | AAGATGGCGAACGAGGCTC | CGCGAGCCAGACAGATTCT | 60.15 | 59.86 | 267 | 71 |
100.00%
|
80.00%
|
| Primer 2000 | Thermotoga | GAAGATGGCGAACGAGGCT | CGCGAGCCAGACAGATTCT | 60.15 | 59.86 | 268 | 71 |
100.00%
|
80.00%
|