| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2411 | Thermotoga | AAGCAGTTCTCGCTCTCGG | CCCACGCGTCTTTCTCGAT | 59.79 | 60.15 | 159 | 63 |
100.00%
|
80.00%
|
| Primer 2412 | Thermotoga | AGCTGCGCTGTGGGATATG | ATGATACCATCGGCACCGG | 60.23 | 59.63 | 210 | 63 |
100.00%
|
80.00%
|
| Primer 2413 | Thermotoga | ATAGCTGCGCTGTGGGAT | ATGATACCATCGGCACCGG | 58.77 | 59.63 | 212 | 63 |
100.00%
|
60.00%
|
| Primer 2414 | Thermotoga | CGTTGTAAGGGCTCACGGT | GACGATGGCAGGAGCGTAT | 60.01 | 59.64 | 205 | 63 |
100.00%
|
80.00%
|
| Primer 2415 | Thermotoga | ATGGAGAGGGTGCTTCGTG | CCAGCACTGAGAAGCGGAT | 59.40 | 59.78 | 289 | 63 |
100.00%
|
80.00%
|
| Primer 2416 | Thermotoga | CCGTGAAGCTCACCCGTAT | CGATGAGAGACCTGGCCTTC | 59.48 | 59.90 | 153 | 63 |
100.00%
|
80.00%
|
| Primer 2417 | Thermotoga | TCGCACTCGATGGCATCA | TTCGGCACCGTTCGCTAT | 59.11 | 59.43 | 287 | 63 |
100.00%
|
40.00%
|
| Primer 2418 | Thermotoga | TCTTCGCACTCGATGGCA | TTCGGCACCGTTCGCTAT | 59.03 | 59.43 | 290 | 63 |
100.00%
|
40.00%
|
| Primer 2419 | Thermotoga | TCCCGTGATGTTTGCCTGT | TCCTTTCCAGCACTGCCAA | 59.55 | 59.46 | 264 | 63 |
100.00%
|
100.00%
|
| Primer 2420 | Thermotoga | ACTGCAAGAAGACGCTCGG | AAACCGGTGTGGGTGCAA | 60.37 | 60.04 | 183 | 63 |
100.00%
|
80.00%
|