| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2421 | Thermotoga | AACAGCGGTGCGTACGTT | AGCGCAACACCACCGTAT | 60.28 | 59.65 | 290 | 63 |
100.00%
|
60.00%
|
| Primer 2422 | Thermotoga | CAACCCCACAAGTTACCGGA | TCCAGCAGATCCAGCACAA | 59.89 | 58.93 | 269 | 63 |
100.00%
|
80.00%
|
| Primer 2423 | Thermotoga | ACAACCCCACAAGTTACCGG | GCAATCCAGCAGATCCAGC | 60.18 | 58.97 | 274 | 63 |
100.00%
|
80.00%
|
| Primer 2424 | Thermotoga | ACAACCCCACAAGTTACCGG | TCCAGCAGATCCAGCACAA | 60.18 | 58.93 | 270 | 63 |
100.00%
|
80.00%
|
| Primer 2425 | Thermotoga | TCCCGTGATGTTTGCCTGT | TCCTTTCCAGCACTGCCAA | 59.55 | 59.46 | 264 | 63 |
100.00%
|
100.00%
|
| Primer 2426 | Thermotoga | AGCGAGAACACACTGGCA | TCCTTCCGTGGTTTTCCGG | 59.18 | 59.93 | 265 | 63 |
100.00%
|
100.00%
|
| Primer 2427 | Thermotoga | TGTGGATGGCTCAGTGGGA | ACAGAATAGCCAGGTGCCC | 60.54 | 59.39 | 187 | 62 |
100.00%
|
40.00%
|
| Primer 2428 | Thermotoga | TGCTGGTCTTGGCGACAT | AAATGCCTCTGCCTTCCCC | 59.25 | 60.00 | 278 | 62 |
100.00%
|
40.00%
|
| Primer 2429 | Thermotoga | AAACGTCACACCGGACTCC | TTCTTCCTCGTCGACGGGA | 59.93 | 60.30 | 157 | 62 |
100.00%
|
80.00%
|
| Primer 2430 | Thermotoga | AGCCGTCATGTCCGTGAA | AGTGCGTGAGTGCCACAA | 58.94 | 59.81 | 230 | 61 |
100.00%
|
60.00%
|