| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2431 | Thermotoga | CCTCATCCCAACGATCGCA | CACAATGGCGAGTCCTGGT | 59.86 | 60.00 | 208 | 61 |
100.00%
|
80.00%
|
| Primer 2432 | Thermotoga | ATCACGCCTCCTGCAGAA | TCGAGCGATCTTACACCGAT | 58.61 | 58.69 | 198 | 61 |
100.00%
|
80.00%
|
| Primer 2433 | Thermotoga | ACGCTCAGGCAAAGTGGA | TCCGAAAACCCACGCGAA | 59.17 | 59.89 | 240 | 61 |
100.00%
|
80.00%
|
| Primer 2434 | Thermotoga | AGTTCTCGGGAGGTCAGCT | CACGCTCACACCGTACTGT | 59.92 | 60.01 | 164 | 61 |
100.00%
|
60.00%
|
| Primer 2435 | Thermotoga | AACGCCATGGCACTTGGA | ACAACGCCAACTGGAAGCT | 59.88 | 60.15 | 290 | 61 |
100.00%
|
80.00%
|
| Primer 2436 | Thermotoga | TGTCTCATGTGCGACGCT | ATCGAAGGCATCAGTCCCG | 59.35 | 59.56 | 230 | 61 |
100.00%
|
80.00%
|
| Primer 2437 | Thermotoga | TGGAGAAGGTGTGGAGGGT | GAACCCAGGTGCCGTTGTA | 59.76 | 59.93 | 267 | 61 |
100.00%
|
60.00%
|
| Primer 2438 | Thermotoga | TGGAGAAGGTGTGGAGGGT | AACCCAGGTGCCGTTGTAG | 59.76 | 59.93 | 266 | 61 |
100.00%
|
60.00%
|
| Primer 2439 | Thermotoga | TGAGGCGTCCGTTTTGCT | TGAAGTTGCTCCCACGGA | 59.89 | 58.44 | 211 | 61 |
100.00%
|
40.00%
|
| Primer 2440 | Thermotoga | TGAGGCGTCCGTTTTGCT | ACAGGCCATCTCGAGGTGT | 59.89 | 60.61 | 187 | 61 |
100.00%
|
40.00%
|