| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 251 | Thermotoga | TCAGTGCACAACCCACCTG | AGCATCGTTTCCGCACCA | 60.15 | 59.97 | 182 | 124 |
100.00%
|
40.00%
|
| Primer 252 | Thermotoga | TACTCAGTGCACAACCCACC | AGCATCGTTTCCGCACCA | 59.89 | 59.97 | 185 | 124 |
100.00%
|
40.00%
|
| Primer 253 | Thermotoga | TTGCGCTCATGCTCTGGT | CAGAGAGGCGTTGTTTGCG | 59.65 | 59.79 | 206 | 124 |
100.00%
|
100.00%
|
| Primer 254 | Thermotoga | AAACGTCGGTGCGGTGAT | ACACGTGCAGGAACACCA | 59.97 | 59.41 | 291 | 124 |
100.00%
|
40.00%
|
| Primer 255 | Thermotoga | CCGAAGCGAGGGGATTCAT | TTGCCAGCACATCGTCCA | 59.55 | 59.57 | 204 | 124 |
100.00%
|
60.00%
|
| Primer 256 | Thermotoga | ACTCAGTGCACAACCCACC | AGCATCGTTTCCGCACCA | 60.15 | 59.97 | 184 | 124 |
100.00%
|
40.00%
|
| Primer 257 | Thermotoga | TCAGTGCACAACCCACCTG | AGCATCGTTTCCGCACCA | 60.15 | 59.97 | 182 | 124 |
100.00%
|
40.00%
|
| Primer 258 | Thermotoga | TACTCAGTGCACAACCCACC | AGCATCGTTTCCGCACCA | 59.89 | 59.97 | 185 | 124 |
100.00%
|
40.00%
|
| Primer 259 | Thermotoga | TGTTTTGCCCAGGTGCCA | ACAGTAACCCAGGAGCTGC | 60.04 | 59.32 | 219 | 124 |
100.00%
|
40.00%
|
| Primer 260 | Thermotoga | TGTTTTGCCCAGGTGCCA | AACCCAGGAGCTGCTTCTC | 60.04 | 59.32 | 214 | 124 |
100.00%
|
40.00%
|