| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 261 | Thermotoga | TGTTTTGCCCAGGTGCCA | CCCAGGAGCTGCTTCTCAAA | 60.04 | 59.96 | 212 | 124 |
100.00%
|
40.00%
|
| Primer 262 | Thermotoga | TTTCGCAGGGGAGAGCATG | ACTGTGCGACGGTGTTGT | 60.08 | 59.81 | 248 | 123 |
100.00%
|
80.00%
|
| Primer 263 | Thermotoga | TTTCGCAGGGGAGAGCATG | ACTGTGCGACGGTGTTGT | 60.08 | 59.81 | 248 | 123 |
100.00%
|
80.00%
|
| Primer 264 | Thermotoga | TGGTGTTGCTCGGCATGT | TCCGTCCGTCAGGTTCATG | 59.89 | 59.41 | 263 | 123 |
100.00%
|
60.00%
|
| Primer 265 | Thermotoga | ACGGTGCTCACAACCAGAG | TACACAGGCGAACGCGTT | 59.93 | 59.97 | 296 | 122 |
100.00%
|
80.00%
|
| Primer 266 | Thermotoga | GCTCACAACCAGAGGGCTT | TACACAGGCGAACGCGTT | 59.93 | 59.97 | 291 | 122 |
100.00%
|
80.00%
|
| Primer 267 | Thermotoga | ACGGTGCTCACAACCAGA | TACACAGGCGAACGCGTT | 58.77 | 59.97 | 296 | 122 |
100.00%
|
80.00%
|
| Primer 268 | Thermotoga | ACGCTCTTGCTCGCTGTT | CCTTCGCGTAGGCTTCACA | 59.97 | 60.08 | 166 | 122 |
100.00%
|
80.00%
|
| Primer 269 | Thermotoga | ACGCTCTTGCTCGCTGTT | ACCCTTCACGATCGCCTTC | 59.97 | 59.78 | 180 | 122 |
100.00%
|
80.00%
|
| Primer 270 | Thermotoga | ACGCTCTTGCTCGCTGTT | GACCCTTCACGATCGCCTT | 59.97 | 59.78 | 181 | 122 |
100.00%
|
80.00%
|