| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 271 | Thermotoga | TTCTTCGCGTGGCTCGTT | CGTTGGTGGGCTCTGTGAA | 59.97 | 60.23 | 247 | 122 |
100.00%
|
80.00%
|
| Primer 272 | Thermotoga | ACCACCACAACCGCAACA | TGTGACGACCGTGTCTCCT | 60.04 | 60.53 | 162 | 122 |
100.00%
|
80.00%
|
| Primer 273 | Thermotoga | ACCACCACAACCGCAACA | ACGACCGTGTCTCCTCGTT | 60.04 | 60.90 | 158 | 122 |
100.00%
|
80.00%
|
| Primer 274 | Thermotoga | TTTCTCGCAGGTTGCCCA | TCGGCAAGTCGGAGTGTT | 59.49 | 58.87 | 192 | 122 |
100.00%
|
40.00%
|
| Primer 275 | Thermotoga | TTACGGCTTGCTCACGCA | GCCAGCACACCAACACCTA | 59.97 | 60.23 | 216 | 122 |
100.00%
|
40.00%
|
| Primer 276 | Thermotoga | TTACGGCTTGCTCACGCA | GCTGTGAGAACGAGAGCCT | 59.97 | 59.41 | 189 | 122 |
100.00%
|
40.00%
|
| Primer 277 | Thermotoga | TCGCTTCGGGAAACGGAA | AGAGACTCTGCCCAGGGTT | 59.26 | 59.84 | 172 | 122 |
100.00%
|
80.00%
|
| Primer 278 | Thermotoga | GGCCTTGGAACACTCGTTG | TCACGCCTCCTCCACTGTA | 59.05 | 59.92 | 153 | 122 |
100.00%
|
80.00%
|
| Primer 279 | Thermotoga | TCATCTCGACCACCAGCAAG | TCACGCCTCCTCCACTGTA | 59.75 | 59.92 | 221 | 122 |
100.00%
|
80.00%
|
| Primer 280 | Thermotoga | CTTCATCTCGACCACCAGCA | TCACGCCTCCTCCACTGTA | 59.75 | 59.92 | 223 | 122 |
100.00%
|
80.00%
|