| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3031 | Thermotoga | AGCAGCGGTGGAATTGCT | CGCACATCACGATCAGGGT | 59.97 | 60.15 | 188 | 50 |
100.00%
|
60.00%
|
| Primer 3032 | Thermotoga | ACACACCACGAACGTGCT | ATCGGCAGCCCTTTCGATC | 59.81 | 60.23 | 169 | 50 |
100.00%
|
60.00%
|
| Primer 3033 | Thermotoga | ACACACCACGAACGTGCT | CGGCAGCCCTTTCGATCAT | 59.81 | 60.52 | 167 | 50 |
100.00%
|
60.00%
|
| Primer 3034 | Thermotoga | ACCAGGTTTGTGGCGAGT | TCGCCTTCAACAACCGCT | 59.08 | 59.89 | 227 | 50 |
100.00%
|
80.00%
|
| Primer 3035 | Thermotoga | GGACTACGTTGACAGGGGAC | TCCACCGTGCTTCTTGCT | 59.75 | 59.17 | 177 | 50 |
100.00%
|
60.00%
|
| Primer 3036 | Thermotoga | GGGGACCGAATTCTCGTGAA | TCCACCGTGCTTCTTGCT | 59.75 | 59.17 | 163 | 50 |
100.00%
|
60.00%
|
| Primer 3037 | Thermotoga | GGCGAGGGAAATGGTGGAA | TGCTGGTGGACATGCAGAG | 60.00 | 60.00 | 280 | 50 |
100.00%
|
80.00%
|
| Primer 3038 | Thermotoga | TTTCTCGACGTCACAGGCC | CACTTCCTTCGGATAGCGGT | 60.01 | 59.54 | 231 | 50 |
100.00%
|
40.00%
|
| Primer 3039 | Thermotoga | CTCCTGCTGGTTCTTGGCT | CCCCAGCCAGAAACGATGT | 59.62 | 60.00 | 274 | 50 |
100.00%
|
60.00%
|
| Primer 3040 | Thermotoga | CTCCTGCTGGTTCTTGGCT | ACCCCAGCCAGAAACGATG | 59.62 | 60.00 | 275 | 50 |
100.00%
|
60.00%
|