| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3041 | Thermotoga | TGGTGTATGGGTGCTCACT | TCCTCCACGGGATGAGAGAA | 58.23 | 59.66 | 183 | 50 |
100.00%
|
40.00%
|
| Primer 3042 | Thermotoga | ACCGCTGATGTCGTAACGG | TGTCCAAGGTCTCCCAGGT | 60.15 | 59.76 | 233 | 50 |
100.00%
|
80.00%
|
| Primer 3043 | Thermotoga | TCCAGACGGGACCCAATGA | TGCCACGTTCGGTTCGAA | 60.23 | 59.89 | 269 | 50 |
100.00%
|
80.00%
|
| Primer 3044 | Thermotoga | AAAACGTGGGACCCATCGG | GTCTGCCGTGAGATGTGGT | 60.30 | 59.71 | 287 | 50 |
100.00%
|
80.00%
|
| Primer 3045 | Thermotoga | TCGAGGAAAACGTGGGACC | GTCTGCCGTGAGATGTGGT | 59.63 | 59.71 | 293 | 50 |
100.00%
|
80.00%
|
| Primer 3046 | Thermotoga | GCCATTCCCGTCTCCGAAA | TTGGGACAGCACCGTGAA | 60.08 | 59.09 | 163 | 50 |
100.00%
|
80.00%
|
| Primer 3047 | Thermotoga | GGAGTGCGGAAGGTGGAAT | AAGTGGCTCGCCTCTTTCC | 59.70 | 60.00 | 250 | 50 |
100.00%
|
100.00%
|
| Primer 3048 | Thermotoga | CGGAAATCTCGGAGCTGGA | TCGAGTCCTCAGGCCAGAA | 59.18 | 59.92 | 263 | 50 |
100.00%
|
80.00%
|
| Primer 3049 | Thermotoga | CGGTCGGACGATCTTCGAA | TGGATGTGGTCGGGTCTCA | 59.57 | 60.23 | 156 | 50 |
100.00%
|
40.00%
|
| Primer 3050 | Thermotoga | CAGACGCCTCTGCACAGAA | TGGCTTGGTGAGTTCTCCG | 60.01 | 59.63 | 204 | 50 |
100.00%
|
60.00%
|