| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3051 | Thermotoga | TCGATCGGATGACGCTTGT | ATGCGGACTGCCAGAGAA | 59.19 | 58.61 | 225 | 50 |
100.00%
|
40.00%
|
| Primer 3052 | Thermotoga | TCGAGCAGGTCCTTCTGGA | AGGTCTTCGGCAGCGAAT | 59.92 | 59.02 | 187 | 50 |
100.00%
|
80.00%
|
| Primer 3053 | Thermotoga | ATCGTTCCTGTGCTCGGTG | AGGTCTTCGGCAGCGAAT | 60.08 | 59.02 | 242 | 50 |
100.00%
|
80.00%
|
| Primer 3054 | Thermotoga | TCGAGCCACAACAGACGAA | CCGCTTCTTCGGGATGAGAA | 59.26 | 59.82 | 236 | 50 |
100.00%
|
80.00%
|
| Primer 3055 | Thermotoga | TCAGGCTGTAACCGTTGCA | TCGGCATTCGCACCAGAA | 59.55 | 59.65 | 213 | 50 |
100.00%
|
40.00%
|
| Primer 3056 | Thermotoga | CGCCAACTTCGACCTCACT | AACTCTTGCGGTCACCAGG | 60.01 | 59.93 | 205 | 49 |
100.00%
|
60.00%
|
| Primer 3057 | Thermotoga | TGCCGTTCTGATACCAGCC | ATCCCAGCCGTTTGCCAT | 59.78 | 59.64 | 199 | 49 |
100.00%
|
80.00%
|
| Primer 3058 | Thermotoga | CTCAGCATCGACCAGGTGT | GAAGGGCTTTCACCGCTCT | 59.41 | 60.00 | 265 | 49 |
100.00%
|
80.00%
|
| Primer 3059 | Thermotoga | ACACGAAGGTTGCGGTTCT | AACCCGTCCTGTTGGAACG | 59.85 | 60.23 | 249 | 49 |
100.00%
|
80.00%
|
| Primer 3060 | Thermotoga | ACACGAAGGTTGCGGTTCT | ACGAGAACCCGTCCTGTTG | 59.85 | 59.63 | 254 | 49 |
100.00%
|
80.00%
|