| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3121 | Thermotoga | AGCGCTCGAAAAGGCAGA | TCCAGCGAATCGTCCAAGG | 59.66 | 59.78 | 243 | 47 |
100.00%
|
40.00%
|
| Primer 3122 | Thermotoga | AGGCCATCAAGAGACACGC | TCGACCGTTGCCTCAACA | 60.08 | 59.18 | 283 | 47 |
100.00%
|
80.00%
|
| Primer 3123 | Thermotoga | TGAAGGTGGTTCAGGACGC | TGTTGGCGTGATCTCCCAC | 59.93 | 60.00 | 216 | 47 |
100.00%
|
80.00%
|
| Primer 3124 | Thermotoga | ACCGAACTCCGCTCTCAGA | GACACCTGCAGCATCCAGA | 60.30 | 59.70 | 190 | 47 |
100.00%
|
80.00%
|
| Primer 3125 | Thermotoga | CGGTTGTCGAATGCGCTT | TGGCACCGGATGGGAAAA | 59.14 | 59.15 | 177 | 47 |
100.00%
|
80.00%
|
| Primer 3126 | Thermotoga | AACGCGAAAAGACACGGC | AAGTCAAGTCCGTCCTCGC | 59.37 | 59.71 | 197 | 47 |
100.00%
|
40.00%
|
| Primer 3127 | Thermotoga | TGCATTCAGGCTCGGTGT | CAGCCTTCTGTCTCCGCTT | 59.25 | 59.71 | 220 | 47 |
100.00%
|
40.00%
|
| Primer 3128 | Thermotoga | ACAGGGCAAGACTCTTCGC | ACACACGTCTCCTGCCTT | 60.00 | 58.45 | 158 | 47 |
100.00%
|
60.00%
|
| Primer 3129 | Thermotoga | TGTTGCGGACGGGTTCTT | TCTTCTGAGCCACTTCCGC | 59.49 | 59.71 | 239 | 47 |
100.00%
|
60.00%
|
| Primer 3130 | Thermotoga | AGCTGGAAGTCCTGGGAGA | TCCATCACAGGTTGCGCA | 59.53 | 59.57 | 167 | 47 |
100.00%
|
40.00%
|