| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3131 | Thermotoga | TTGTCACGCTGATCGCCA | ACGCAAGCCAGCTGAAGA | 59.66 | 59.57 | 210 | 47 |
100.00%
|
80.00%
|
| Primer 3132 | Thermotoga | TGTCACGCTGATCGCCAT | ACGCAAGCCAGCTGAAGA | 59.42 | 59.57 | 209 | 47 |
100.00%
|
80.00%
|
| Primer 3133 | Thermotoga | CCTGGCGTCTTGTGAAGGA | TCAGCGTGTGGGCTTCTT | 59.63 | 59.17 | 183 | 47 |
100.00%
|
80.00%
|
| Primer 3134 | Thermotoga | CCAGCACGAAAGATCCCCA | ACGTGTCGTTCGACAGCTT | 59.70 | 59.93 | 294 | 47 |
100.00%
|
80.00%
|
| Primer 3135 | Thermotoga | ACCAGCACGAAAGATCCCC | ACGTGTCGTTCGACAGCTT | 59.70 | 59.93 | 295 | 47 |
100.00%
|
80.00%
|
| Primer 3136 | Thermotoga | AGCTCAGGAAGAGGCCAGA | TCCACTGCTGACTCGTCCA | 59.92 | 60.53 | 288 | 47 |
100.00%
|
80.00%
|
| Primer 3137 | Thermotoga | AGCTCAGGAAGAGGCCAGA | CCACTGCTGACTCGTCCAT | 59.92 | 59.41 | 287 | 47 |
100.00%
|
80.00%
|
| Primer 3138 | Thermotoga | GCTGTCGAAAGCGCATCA | ACGAGACCACCATCCGAGA | 58.83 | 60.00 | 188 | 47 |
100.00%
|
80.00%
|
| Primer 3139 | Thermotoga | TCTTGCCGTGAAGCTCACC | CGATGAGAGACCTGGCCTT | 60.30 | 58.79 | 158 | 47 |
100.00%
|
80.00%
|
| Primer 3140 | Thermotoga | TGAAGCTCCAGGTACCCAGA | ATTCGCGACAGCCGACTT | 59.88 | 59.74 | 254 | 47 |
100.00%
|
80.00%
|