| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3151 | Thermotoga | ACAGCCAGAAGAACGGTCG | TCATAGCGTGGAACTCCGC | 60.01 | 59.86 | 265 | 46 |
100.00%
|
60.00%
|
| Primer 3152 | Thermotoga | CGCGATGCACAGAGATCCA | ACCTCAACCAGAACGAGCG | 60.23 | 60.01 | 213 | 46 |
100.00%
|
80.00%
|
| Primer 3153 | Thermotoga | AGCGCACGGAGTTGTTCT | AAGCGAGGAGAGAACAGCG | 59.58 | 59.79 | 220 | 46 |
100.00%
|
60.00%
|
| Primer 3154 | Thermotoga | GGCTTTGATGCACAGACCG | GCAACGCGATCGCAAAGT | 59.50 | 59.83 | 174 | 46 |
100.00%
|
40.00%
|
| Primer 3155 | Thermotoga | TCCGTTGAGGTGCAGATCG | CCGGTTTCTTGTGTCTCAGGT | 59.78 | 60.20 | 157 | 46 |
100.00%
|
80.00%
|
| Primer 3156 | Thermotoga | TGCTCCACAACACCGTGA | ACCTCCGTAGTTGAACGCG | 59.10 | 60.08 | 296 | 46 |
100.00%
|
40.00%
|
| Primer 3157 | Thermotoga | ACCGGTTTCCATGCTGGA | CCGTCGTCGTTGGTTACCA | 58.83 | 60.01 | 298 | 46 |
100.00%
|
80.00%
|
| Primer 3158 | Thermotoga | TGCTCAAGTTGGAGGCCAA | TCGGGTGTTTTCTGCGTCG | 59.46 | 60.95 | 181 | 46 |
100.00%
|
80.00%
|
| Primer 3159 | Thermotoga | ACGGCTAATCTCGGATGCG | GGCTTTCATGTCGTTCGGC | 60.01 | 59.87 | 255 | 46 |
100.00%
|
80.00%
|
| Primer 3160 | Thermotoga | TCCGGTGAACGTGATAGCG | AAACACCCTGGAGAGTGCC | 59.86 | 59.54 | 298 | 46 |
100.00%
|
40.00%
|